压力诱导大鼠颈总动脉即刻早基因产物c-Jun的表达  

Pressure-Induced Expression of Immediate-Early Gene Product c-Jun of the Common Carotid Arteries in Rats

作　　者：严志强[1] 姜宗来[1] 张炎[2] 杨向群[2] 

机构地区：[1]上海交通大学医学院力学生物学与医学工程实验室,上海200030 [2]第二军医大学解剖学教研室,上海200433

出　　处：《生物医学工程学杂志》2005年第1期35-37,共3页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目 (10 13 2 0 2 0 )

摘　　要：为了探讨压力在动脉重建中的作用机制 ,研究压力对动脉即早基因产物 c- Jun表达的影响。采用大鼠颈总动脉在体受力模型 ,以高压力 (16 0 mm Hg)和正常压力 (80 mm Hg)灌流颈总动脉 0 .5、1、3、6 h,免疫组织化学和计算机图像处理检测颈总动脉 c- Jun的表达。结果显示 ,正常压力作用下 ,1h组颈总动脉 c- Jun有少量表达 ,3h和6 h组有所增加。高压力作用 0 .5 h即可诱导颈总动脉 c- Jun的表达 ,随着时间的延长 ,c- Jun的表达与正常压力组相比有明显增加 (P<0 .0 1)。结果提示 ,压力可以诱导动脉 c- Jun的表达 ,这一机制可能在压力诱导动脉重建中起重要作用。In order to investigate the mechanism of mechanical stress-mediated arterial remodeling, we studied the pressure-induced expression of immediate-early response gene product c-Jun in common carotid arteries in rats. The common carotid arteries were perfused with both high pressure （160 mmHg） and normal pressure （80 mmHg） for 0. 5, 1, 3 and 6 hours. Expression of immediate-early response gene product c-Jun in the arteries was examined by immunohistoehemistry and computer image processing, c-Jun was weakly expressed at 1 h, then increased at 3 h and 6 h after exposure of the arteries to normal pressure. Positive immonohistochemical product of c-Jun appeared in the arteries at 0.5 h after the onset of high pressure, then it increased markedly till 6 h. There was significant difference between the two groups. These results indicate that expression of c-Jun of the arteries can be induced by pressure, which may play an important role in mechanical stress-mediated arterial remodeling.

关 键 词：压力 大鼠 颈总动脉 动脉即刻早基因产物 C-JUN 基因表达 动脉重建 

分 类 号：R318.01[医药卫生—生物医学工程]