电脉冲致离体S-180细胞电穿孔的观察与研究  

Observation and Research on the Electroporation of S-180 Cell Induced by Electric Pulse

作　　者：李虹[1] 耿芹[2] 岳碧松[1] 邹方东[1] 王保义[3] 张弘[3] 曾浩[3] 

机构地区：[1]四川大学生命科学院,成都610064 [2]四川出入境检验检疫局,成都610041 [3]四川大学无线电系,成都610064

出　　处：《生物医学工程学杂志》2005年第1期47-49,共3页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助 (60 0 710 2 4);四川省科技厅基金资助(0 2 SY0 2 9-12 7)

摘　　要：扫描电镜下我们观察到了一定强度电脉冲致 S- 180细胞电穿孔的现象。同时分别改变电压、电容、脉冲个数 ,对 S- 180离体细胞进行电脉冲作用 ,通过苔盼蓝追踪 ,发现在一定的条件下 ,随着对 S- 180离体细胞电穿孔的电压越大、电容越小、脉冲个数越多 ,其穿孔百分率越大。This is our first time report of S-180 cell handled with various voltages, capacity and pulse number. Many pores on the S-180 cell membrane can be observed under scanning electron microscope. By the stains of trypan blue, we have known the influence of electric parameters on the ratio of poration, The results show that the ratio of electroporation has positive correlation with the voltages and the pulse number while negative correlation with the capacity.

关 键 词：电脉冲 离体细胞 S-180细胞 电穿孔 扫描电镜 红细胞 电磁脉冲 

分 类 号：Q27[生物学—细胞生物学]