全生物化组织工程血管构建的初步研究  

Primary Research of Reconstruction of Complete Biological Tissue Engineered Blood Vessel

作　　者：张炎[1] 刘太华[1] 姜宗来[2] 张秀花[1] 刘波[1] 李玉泉[1] 

机构地区：[1]第二军医大学解剖学教研室,上海200433 [2]上海交通大学医学院生物力学实验室,上海200030

出　　处：《生物医学工程学杂志》2005年第3期448-451,共4页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(10272110;10132020)

摘　　要：为探索在体外初步构建全生物化组织工程血管,采用以酶消化为主的方法制备猪颈总动脉脱细胞支架,再种植犬胸主动脉的平滑肌细胞,培养四周。经组织学染色和电镜观察显示:在猪颈总动脉脱细胞支架上,犬血管平滑肌细胞大量生长,组织学和电镜结构与正常血管壁结构类似。结果表明,我们初步成功地构建了全生物化组织工程血管,为临床血管替代物的基础研究提供了有意义的实验资料。To explore primarily the reconstruction of biological tissue engineered blood vessel in vitro, the decellularised scaffolds were obtained from swine common carotid arteries by enzyme digestion, and vascular smooth muscle cells （VSMCs） were isolated from canine thoracic aorta and subcultured and purified. The VSMCs were seeded into the inner surface of scaffolds and they were cocultured in vitro for 4 weeks. Histological staining and transmission electron microscopy were used to observe the growth of canine VSMCs on swine decellularized arterial scaffolds. The results showed the seeded VSMCs grew well on the decellularized scaffold throughout the duration of 4 weeks, which suggests that the method in this article is practicable for reconstructing complete biological tissue engineering blood vessel.

关 键 词：全生物化组织工程 血管构建 脱细胞动脉支架 血管替代物 血管壁 

分 类 号：R318.0[医药卫生—生物医学工程]