用表面界面性能评价生物碳素材料血液相容性的可行性研究  

A Feasibility Study on Use of Surface and Interface Properties for Evaluating Hemocompatibility of Carbonaceous Biomaterials

作　　者：李伯刚[1] 康安福[1] 尹光福[1] 郑昌琼[1] 

机构地区：[1]四川大学材料科学与工程学院,成都610065

出　　处：《生物医学工程学杂志》2005年第3期452-455,共4页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(39870232);教育部博士点基金资助项目(200061004)

摘　　要：以类金刚石薄膜(DLC)、含硅DLC、石墨、金刚石薄膜(DF)、低温各向同性碳(LTIC)和碳化硅为材料样本,用T型关联度法对其动态凝血时间、血小板消耗率、溶血率和表面能量参数等数据进行了灰色关联分析。结果显示:(1)动态凝血时间与亲水性的表面能量参数呈正相关,与临界表面张力呈负相关,其中与界面张力的关联度最大(0.63),其次为临界表面张力(-0.43);(2)与动态凝血时间的情形刚好相反,溶血率与亲水性的表面能量参数呈负相关,而与临界表面张力呈正相关,对溶血率影响较大的仍然是界面张力(-0.43)和临界表面张力(0.29);(3)与血小板消耗率关联最大的是临界表面张力(0.68),其次为表面张力(0.32);(4)动态凝血时间与血小板消耗率、溶血率之间有较大的负关联度。结果表明:动态凝血时间取决于其表面亲水性和有限润湿的平衡,血小板消耗的基础是材料表面良好的润湿与亲水性,而材料表面的疏水作用和充分的润湿则成为溶血的推动力量;动态凝血时间与溶血率和血小板消耗率之间具有“跷跷板效应”,因而可等效地用动态凝血时间评价生物碳素材料的血液相容性。证实:以血浆蛋白吸附为先导的血小板黏附、变形而致血栓形成,是生物碳素材料的主要凝血机制;可以临界表面张力为指标评价其血液相容性。本研究为用表面界面性能评价生物碳素材料的血液相容性提供了理论依据。In order to evaluate diamond like carbon film（DLC）, DLC containing Si,graphite, diamond film （DF）, low temperature isotropic carbon film （ LTIC）and SiC,we investigated the correlations between surface energy parameters and hemocompatibility indices such as kinetic clotting time, hemolysis and platelet consumption. An analysis of T-type correlation degree in the Grey system theory was performed. The results showed： （1） all of correlation degrees between kinetic clotting time and polar surface energy parameters were positive, but for critical surface tension, the correlation degree was negative ; among five of surface energy parameters, interface tension had the highest relation degree （0. 63） with kinetic clotting time, and critical surface tension （-0.43） took the second place; （2） on the contrary, all of correlation degrees between hemolysis and polar surface energy parameters were negative, but for critical surface tension,the correlation degree was positive; and that which had closer correlations with hemolysis were still interface tension （-0.43） and critical surface tension （0.29） ; （3） critical surface tension had the highest relation degree （0. 68） with platelet consumption, and surface tension （0. 32） took the second place; （4） kinetic clotting time possessed higher negative correlation degrees with hemolysis （- 0. 57） and platelet consumption （- 0. 36）. These data indicate that kinetic clotting time depended on a balance between consumption was the polarity of surface and the limited humidifying of water on the surface, and that platelet based on good hum：dification and power polarity of surface, while hemolysis was promoted by the aid of chromatic dispersion action stemming from the surface and fully humidifying of water on the surtace, lhere was ＂seesaw effect＂ between kinetic clotting time and hemolysis or platelet consumption, hence the hemocompatibility of carbonaceous biomaterials could be equivalently evaluated by using kinetic c

关 键 词：表面界面性能 生物碳素材料 血液相容性 凝血机制 血栓形成 灰色关联分析 

分 类 号：R318.08[医药卫生—生物医学工程]