人脐静脉内皮细胞在RGD肽聚酯材料表面的黏附稳定性研究  

Effect of RGD Peptide on Adhesive Stability of Human Umbilical Vein Endothelial Cell on Polyethylene Terephthalate Surface

作　　者：武忠[1] 赁可[1] 石应康[1] 万昌秀[2] 赵强[2] 

机构地区：[1]四川大学华西医院胸心外科 [2]四川大学高分子材料与工程系,成都610065

出　　处：《生物医学工程学杂志》2005年第3期456-458,共3页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(29874022)

摘　　要：研究RGD肽对内皮细胞(Endothelialcell,EC)在生物材料表面黏附稳定性的影响。实验材料(聚酯)分为三组:RGD组(表面共价接枝人工合成的RGD三肽)、对照组(表面预衬纤维粘连蛋白)和空白组(表面未作任何处理),然后在三组材料表面种植体外培养的人脐静脉内皮细胞,并在定常流条件下观察比较RGD肽和纤维粘连蛋白对材料表面细胞黏附稳定性的影响。结果显示随着剪切力作用时间延长和剪切力加大,三组材料表面黏附的细胞脱落逐渐增多。空白组PET表面细胞脱落最为明显,8.19dyne/cm2作用4h后,材料表面细胞残留率仅为13.73%。PET表面结合RGD或纤维粘连蛋白后,细胞残留率明显增加,同样剪切力作用下细胞残留率分别为43.33%和40.75%,两组之间无显著性差异。由此得出结论,RGD可以提高EC在材料表面的黏附稳定性。本结果仅是一个体外实验的初步结果,需要进一步的体内实验加以证实。In this study for exploring the effect of RGD peptide on adhesive stability of endothelial cells on biomaterial surface, all materials were divided into three groups： RGD group （ PET covalently grafted with synthetic RGD peptides）, control group （ PET precoated with fibronectin） and blank group （Non-coated PET surface）. Cultured human umbilical vein endothelial cells （HUVECs） were seeded on the materials, then the adhesive stability of HUVECs on the varied PET surfaces was observed under steady flow condition, and the effects of shear stress and shear time on adherent cells were compared. The results showed that the resistance of adherent endothelial cells to detachment by flow was shear stress and shear time dependent. Comparison of the three groups under the same condition revealed that the ECs retention rates of RGD-grafted or fibronectin-coated group were much higher than that of the non-coated group. Under 8.19 dyne/cmz shear stress after 4h, ECs retention rates were 13. 73 % （blank group）, 43. 33 % （RGD group） and 40. 750% （control group） respectively. These data indicated that RGD peptide can improve the adhesive stability of endothelial cell on biomaterial surface and the effect of RGD in vivo needs further studies.

关 键 词：人脐静脉 内皮细胞 RGD肽聚酯材料 黏附稳定性 材料表面 生物材料 

分 类 号：R318.08[医药卫生—生物医学工程]