磺化对聚醚砜膜片吸附β_2-微球蛋白的影响  

Effect of Sulfonation of Polyethersulfone Sheets on the Adsorption of β_2-Microglobulin

作　　者：程莉萍[1] 孙树东[2] 乐以伦[2] 黄嘉[2] 毛华毅[3] 梁博[2] 

机构地区：[1]四川大学材料学院,成都610065 [2]四川大学高分子科学与工程学院,成都610065 [3]中国核动力研究设计院成都同位素应用研究所,成都610041

出　　处：《生物医学工程学杂志》2005年第3期459-462,共4页Journal of Biomedical Engineering

基　　金："九五"国家科技攻关项目(97-564-01-05)

摘　　要：研究了不同磺化度的聚醚砜血液透析膜材在体外静态接触人血清时吸附去除其中的β2-微球蛋白(β2-microglobulin,β2M)的能力。采用放免法测定了膜分别在125I-β2溶液和在人血清中,在37℃孵育不同时间后对125I-β2M和β2M的吸附进行了对比分析。结果:膜材对125I-β2M的饱和吸附百分率由大到小依次是PES-SO3Na->PES-SO3Na->>PES;但在血清孵育体系中,不同材料对β2M的吸附均在30min达到最大吸附值,最后稳定的吸附量由大到小依次是PES-SO3Na->PES-SO3Na->PES。说明通过用膜吸附方式去除β2M是一种有效的方法。对PES进行磺化改性可大大提高材料对β2M的吸附去除量,而且随着磺化度增加,膜吸附β2M的量增加。本研究合成出的PES-SO3Na可能用于去除患者体内的β2M和降低、延缓透析相关淀粉样变性病的发生。This study was performed to evaluate the adsorption of β2-microglobulin（β2 M） by blood dialysis membrane materials which are polyethersulfone （PES）, sulfonated polyethersulfones, （PES-SO3Na-I and PES- SO3Na- Ⅱ ） in vitro incubated in human serum and radiolabeled β2M （125I-β2 M） solution respectively. In these experiments, the materials were incubated in ^125I-β2 M solution and human serum at the appointed time ranging from 15 minutes to four hours at 37℃, and then the amounts of ^125I-β2M and serum β2M adsorbed by materials were measured by radioimmunoassay （RIA）. In the ^125I-β2M system, amounts of ^125I-β2M adsorbed by the materials decreased in sequence of PES-SO3 Na-Ⅱ〉PES-SO3Na-I〉〉PES. In the serum system, amounts of β2M adsorbed reached maximums at 30 minutes and the final adsorptions decreased in sequence of PES-SO3Na-II PES-SO3Na-I〉PES. Sulfonated PES removes β2M more than PES does and the adsorption of β2M increases with the increase in the degree of sulfonation. Its ability to remove significant amount of β2M may result in less β2M available for incorporation into amyloid. The use of PES-SO3Na membranes lessens the likelihood of dialysisrelated amyloidosis （DRA） development, which remains a major source of morbidity for patients treated with longterm hemodialysis.

关 键 词：聚醚砜 膜片吸附 Β2-微球蛋白 磺化度 血液透析 

分 类 号：R318.08[医药卫生—生物医学工程]