外消旋聚乳酸与软骨细胞体外培养的实验研究  

Culture of Chondrocytes onto Poly-DL-Lactide Scaffoldings: Experimental Study

作　　者：彭智[1] 宋跃明[1] 王定国[2] 刘立岷[1] 丁永利[1] 

机构地区：[1]四川大学华西医院骨科 [2]四川省劳动卫生职业病防治研究所,成都610041

出　　处：《生物医学工程学杂志》2005年第3期518-520,共3页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(39670224)

摘　　要：探讨国产外消旋聚乳酸(Poly-DL-Lactide,PDLLA)材料作为软骨组织工程技术中细胞培养支架的可行性及不同孔隙率的PDLLA支架对软骨细胞吸附增殖的影响.采用不同孔隙率的PDLLA支架与软骨细胞体外培养,观察其亲水性的改变,细胞吸附及对细胞增殖的影响.软骨细胞在不同规格的PDLLA支架上均能生长增殖分泌基质.孔隙率在90%～96%的PDLLA支架细胞吸附、增殖较佳.This experimental study was intended to find the possibility of using PDLLA as scaffolds in tissue engineering and to assess the effect of PDLLA scaffolds with different porosity on chondrocytes. Chondrocytes were cultured when they were seeded onto PDLLA scaffolds with different porosity. Under scanning electron microscope, the scaffolds in hydrophila and adsorptivity to chondrocytes and the function of the cells were observed. The chondrocytes were noted to proliferate on PDLLA scaffolds with different porosity, those with 90%-96% porosity being significantly better. So PDLLA can he used as a scaffold in tissue engineering, and the proper porosity of PDLLA scaffolds should he 90%-96%.

关 键 词：外消旋聚乳酸 软骨细胞 体外培养 软骨组织工程 细胞培养支架 细胞吸附 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]