5-氟尿嘧啶-花生凝集素结合物的制备与结合率测定  

Preparation of 5-Fluorouracil-peanut Agglutinin Conjugate and Determination of the Ratio of Drug to Conjugate

作　　者：蔡勤[1] 孟延发[2] 张志荣[1] 

机构地区：[1]四川大学华西药学院,成都610041 [2]四川大学生命科学学院,成都610065

出　　处：《生物医学工程学杂志》2005年第3期545-547,共3页Journal of Biomedical Engineering

基　　金：国家杰出青年科学基金资助项目(39925039)

摘　　要：采用碳二亚胺法将经过羧基化衍生的5-氟尿嘧啶连接到花生凝集素上,制备一种导向肿瘤的5-氟尿嘧啶-花生凝集素结合物,并应用改进的三硝基苯磺酸法测定药物结合率。测得的药物结合率为76.33%。结果表明碳二亚胺法可成功实现药物与花生凝集素的连接,该法制备得到的结合物其药物结合率高。5-fluorouracil was combined with peanut agglutinin by a water-soluble carbodiimide to prepare the tumor target coniugate of 5-fluorouracil-peanut agglutinin and the ratio of drug to conjugate was determined by the modified trinitrobenzenesulfonic acid method （TNBS）. The ratio of drug to conjugate was 76. 33%. The result showed that 5-fluorouracil could link to the peanut agglutinin by EDC/NHS crosslinking with high drug ratio.

关 键 词：5-氟尿嘧啶 花生凝集素 制备工艺 含量测定 碳二亚胺法 药物结合率 抗肿瘤药物 

分 类 号：TQ463.54[化学工程—制药化工]