肾上腺髓质素对正常Wistar大鼠胰岛素敏感性的影响  

Effect of adrenomedullin on insulin sensitivity in normal Wistar rats

作　　者：李华琦[1] 童南伟[1] 

机构地区：[1]四川大学华西医院内分泌科,成都610041

出　　处：《中华内分泌代谢杂志》2005年第5期467-468,共2页Chinese Journal of Endocrinology and Metabolism

基　　金：2002年度四川省学术带头人培养基金资助项目

摘　　要：用高胰岛素正常血糖钳夹试验,联合放射性同位素稀释示踪技术观察肾上腺髓质素(AM)对正常Wistar大鼠胰岛素敏感性的影响。输入生理盐水及0.05、0.2、1μg·kg-1·min-1等不同剂量的AM,测定葡萄糖利用率(GDR)、肝脏葡萄糖输出率(HGO)。AM可使正常Wistar大鼠胰岛素刺激下的GDR值下降,并有剂量依赖趋势,尤以1μg·kg-1·min-1最明显(下降51%),可能还有增加HGO的作用,提示较高浓度的AM可导致以外周为主的胰岛素抵抗的发生。The effect of adrenomedullin （AM） on insulin sensitivity in normal Wistar rats was investigated by hyperinsulinemic-euglycemic clamp in combination with isotope dilution technique. The infusion rates of AM during the clamp were 0, 0.05, 0.2,1 μg·kg^-1·min^-1, rspectively. The glucose disposal rate （GDR） and hepatic glucose output （HGO） were calculated by the plasma 3-3 H-glucose specific activity. After AM infusion, the insulin-mediated glucose disposal was reduced in a dose-dependant manner. It indicated that high dose of AM could induce a marked state of insulin resistance, which was mainly characterized by inhibition of the effect of insulin to stimulate peripheral glucose metabolism.

关 键 词：肾上腺髓质素 WISTAR大鼠 胰岛素敏感性 胰岛素抵抗 嗜铬细胞瘤 胰岛素钳夹术 

分 类 号：R587.1[医药卫生—内分泌]