兔腹主动脉狭窄后剪切力的变化对局部内皮细胞形态和通透性的影响  

Effects of Wall Shear Stress on the Morphology and Permeability of Endothelial Cells in Stenotic Rabbit Abdominal Aorta

作　　者：吴岩[1] 邓小燕[1] 曾宪敏[2] 王珂[3] 

机构地区：[1]重庆大学生物力学与组织工程教育部重点实验室,重庆400044 [2]本钢总医院心内科,本溪117000 [3]大连医科大学附属第一医院心内科,大连116023

出　　处：《生物医学工程学杂志》2005年第2期225-229,共5页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目 (10 172 0 2 7) ;国家教育部重点实验室资助项目 (97-0 2 84)

摘　　要：兔腹主动脉狭窄至正常的5 5 .2 % ,伊文思蓝对动脉染色,在扫描电镜下观察内皮细胞形态,计算细胞形态指数。动脉狭窄血管内流动壁面剪切率的分布由计算机数值模拟确定。结果表明,在紧接狭窄处的近远两侧,血流受到严重干扰,内皮细胞的形态和对伊文思蓝白蛋白复合物通透性发生明显改变。研究表明,这些改变不但与剪切应力的大小有关,还与血液流动的状态有关。这与动脉粥样硬化一般好发于血液流动受到干扰,流动发生分离区域的解剖观察一致。Stenosis with 55.2% cross section area reduction was introduced into the rabbit aorta. Using Evans blue dye and scanning microscope, we observed the morphology of endothelial cells and the permeability of endothelium to albumin in the stenotic aorta. Numerical simulation of blood flow in the stenotic aorta was performed to obtain the distribution of wall shear rate. The results showed that in the immediately proximal and distal vicinity of stenosis, blood flow was disturbed significantly, resulting in apparent changes in the morphology of endothelial cells and the permeability of endothelium to albumin. These changes were not only attributed to the value of wall shear stress, but also attributed to the flow pattern in the stenosis. The result therefore is in good consistent with the clinical observation that atherosclerosis often occurs in the areas where blood flow is disturbed and flow separation occurs.

关 键 词：狭窄 内皮细胞形态 通透性 壁面剪切力 腹主动脉狭窄 剪切力 计算机数值模拟 局部 动脉粥样硬化 兔 

分 类 号：R543.3[医药卫生—心血管疾病]