IL-8在兔动脉粥样硬化模型中斑块处的蛋白和基因表达  

Interleukin-8 Protein and Gene Expression in Atherosclerotic Lesions of Hyperlipemia Rabbits

作　　者：聂永梅[1] 陈槐卿[1] 成敏[1] 刘小菁[2] 邓伊伶[3] 

机构地区：[1]四川大学华西医学中心生物医学工程研究室,成都610041 [2]四川大学华西医院心血管疾病研究室,成都610041 [3]四川大学华西医院病理研究室,成都610041

出　　处：《生物医学工程学杂志》2005年第2期258-264,共7页Journal of Biomedical Engineering

摘　　要：白细胞介素- 8(Interleukin- 8,IL- 8)是趋化因子CXC亚家族的一员,对中性粒细胞有趋化作用,可以诱导平滑肌细胞的增殖和迁移,是一个促血管生成因子,在动脉粥样硬化慢性炎症过程中起着重要作用。本文通过建立兔高脂血症动脉粥样硬化斑块模型,动态监测了在动脉粥样硬化发展过程中IL- 8的蛋白和基因表达,以了解IL- 8在动脉粥样硬化中的作用。采用免疫组化的方法定位IL- 8在高脂血症兔动脉粥样硬化斑块模型主动脉升弓部血管壁的蛋白表达,8、12周模型组内膜显著增厚并有明显阳性染色,半定量分析AS模型组阳性染色的面积分别是对照组的4 .4 8、8.76倍,平均积分光密度(IOD)值分别是对照组的4 .16、4 .36倍。采用双抗夹心EL ISA法测定兔血管组织匀浆液中的IL- 8蛋白表达量,8、12周AS模型组IL- 8蛋白含量与总蛋白比值和对照组比较均有显著性差异,分别是对照组的1.84、2 .0 6倍。用原位杂交的方法定位IL- 8m RNA的表达,8周模型组血管内膜有强的阳性染色,IL- 8m RNA的表达增加。本研究通过建立动脉粥样硬化模型,动态监测了整个动脉粥样硬化过程中IL-8的蛋白和基因表达。IL-Interleukin-8 is CXC chemokine that is initially discovered using chemotaxis and the activation of neutrophils and induces the migration and proliferation of smooth muscle cells. Interleukin-8 is a potent angiogenic factor that may play a role in atherosclerosis. To establish the temporal correlation between IL-8 expression and plaque development, we examined the expression during atherosclerosis of hyperlipemia rabbits using immunohistochemistry, ELISA, in situ hybridization. By location of immunohistochemistry, the expression of IL-8 protein increased obviously in intima of hyperlipemia rabbits at 8 and 12 week. Quantitative analysis of the expression of IL-8 Immunohistochemistry indicated that positive area of AS model was 4.48 times and 8. 76 times that of control group at 8 and 12 week. The valuation of IOD of AS model was 4.16 times and 4.36 times that of control group at 8 and 12 week. By specific ELISA ,the ratio of the IL-8 protein to total protein of AS model was 1.84 times and 2.06 times that of control group at 8 and 12 week. By location of in situ hybridization, positive location was strong in intima of hyperlipemia rabbits at 8 week. We observed the dynamic alteration of interleukin-8 protein and gene expression in atherosclerotic lesions of hyperlipemia rabbits with establishing model. Interleukin-8 protein and gene expression was up-regulation in the development of fatty streaks in hyperlipemia rabbit.

关 键 词：白细胞介素-8 动脉粥样硬化 免疫组化 ELISA 原位杂交 动脉粥样硬化模型 IL-8MRNA 动脉粥样硬化斑块 蛋白表达 基因表达 

分 类 号：R543.5[医药卫生—心血管疾病]