汽油和甲醇两种燃料的汽车尾气的TK基因突变试验比较  

Genotoxicity Comparison between Gasoline- and Methanol-fueled Exhaust by TK Gene Mutation Assay

作　　者：梁英[1] 詹立[2] 张遵真[1] 张浩[1] 曾详贵[1] 苟小静[3] 林川[3] 蔡春华[3] 邵茜[1] 邵国祥[1] 吴德生[1] 

机构地区：[1]四川大学华西公共卫生学院 [2]四川大学华西医院国家成都中药安全性评价中心,成都610041 [3]中国石油西南油气田分公司川西北气矿,江油621709

出　　处：《生物医学工程学杂志》2005年第2期347-350,共4页Journal of Biomedical Engineering

基　　金：中国石油西南油气田分公司川西北气矿资助项目(XNS0 5 JS2 0 0 3 -82 ) ;四川大学校青年基金资助项目(0 0 3 0 5 0 5 5 0 5 0 11)

摘　　要：甲醇燃料极有希望成为更清洁的汽油替代品,然而有关甲醇燃料汽车尾气对健康影响的研究报道极少,更未见对甲醇和汽油两种燃料汽车尾气的遗传毒性进行比较研究。为此本课题在同样的车型,同样的工况条件下采集了甲醇尾气和汽油尾气,选用L 5 178Y细胞Thymidine kinase(TK)基因突变试验,在同样的剂量范围进行了两种尾气的遗传毒性检测,并与微核试验和彗星试验的结果进行了比较。结果显示,在33～133ml/ m l范围内,汽油尾气的遗传毒性强于甲醇尾气,但是甲醇尾气的细胞毒性强于汽油尾气;与微核试验和彗星试验比较证实,L 5 178Y细胞TK基因试验检测基因突变更加灵敏。Methanol fuel is a most promising substitute for gasoline. It is scarcely reported about methanolfueled exhaust on the health effect,neither about genotoxicity research between methanol- and gasoline-fueled exhaust. In the present study, the two kinds of exhaust were sampled directly from tailpipe at the same type bus, the same state, L5178Y thymidine kinase（TK） gene mutation assay was used to investigate their genotoxicity at the same dose range,and compared with micronucleus and comet assay. The results showed that the genotoxicity of gasoline-fueled exhaust is stronger than that of methanol-fueled exhaust,while the cytotoxicity of methanol-fueled exhaust is stronger than that of gasoline-fueled exhaust at dose range. The study demonstrated that L5178Y TK gene mutation assay is more sensitive than micronucleus and comet assay.

关 键 词：甲醇尾气 汽油尾气 TK(Thymidine kinase)基因 遗传毒性 TK基因突变试验 汽车尾气 甲醇 汽油 燃料 

分 类 号：X734[环境科学与工程—环境工程]