淋球菌多重耐药性与mtrR基因点突变的关系  

Relationship between Multi-drug Resistant Neisseria gonorrhoeae and Point Mutation of mtrR Gene

作　　者：陈宏翔[1] 涂亚庭[1] 林能兴[1] 黄长征[1] 张丽霞[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院皮肤性病科,武汉430022

出　　处：《华中科技大学学报（医学版）》2005年第5期616-618,621,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：国家自然科学基金资助项目(No.30371293)

摘　　要：目的探讨多传递耐药(mtr)外排系统中mtrR基因点突变与淋球菌流行株多重耐药性的关系。方法应用K-B法和琼脂稀释法分离出55株淋球菌流行株。利用小量细菌基因组DNA快速抽提试剂盒取淋球菌DNA,PCR扩增16株淋球菌mtrR基因,并对扩增产物测序,比较淋球菌敏感株与多重耐药株的差异。结果淋球菌多重耐药株占76.36%(42/55)。4株敏感株和2株耐青霉素淋球菌无mtrR基因突变,10株多重耐药株均有mtrR基因点突变,表现为3种点突变形式:6株发生了第45位Gly(GGC→GAC)Asp,3株发生了第14位His(CAC→CGC)Arg,1株发生了第51位Phe(TTC→GTC)Val。结论淋球菌mtrR基因第45位Gly(GGC→GAC)Asp、14位His(CAC→CGC)Arg和第51位Phe(TTC→GTC)Val突变与淋球菌流行株的多重耐药性密切相关。Objective To investigate the relationship between the multi-drug resistant Neisseria gonorrhoeae and the point mutation of rntrR gene of mtrRCDE efflux system. Methods A total of 55 multi-drug resistant strains were obtained by the K-B and agar dilution methods. The chromosome DNA was extracted using little dose bacterial genome DNA extracting kit. The mtrR gene of Neisseria g onorrhoeae was examined by polymerase chain reaction （PCR）, and the PCR product sequences were detected to test the difference between the sensitive and resistant strains. Results The percentage of multi-drug resistant strain was accounted for 76.36% （42/55）. There was no mtrR mutation in 4 sensitive and 2 penicillin-resistant strains. The point mutation of 10 multi-drug resistant strains occurred in three positions of mtrR gene： 6 strains in the position 45 Gly （GGC→GAC） Asp, 3 strains in the position 1,1 His （CAC→CGC） Arg and 1 strains in the position 51 Phe （TTC→GTC） Val. Conclusion The mutations in the position 45 Gly （GGC→GAC） Asp, 14 His （CAC→CGC） Arg and 51 Phe （TTC→GTC） Val have a close relation with the multi-drug resistance of Neisseria gonorrhoeae.

关 键 词：奈瑟氏淋球菌 淋病 药物耐受性 

分 类 号：R378.1[医药卫生—病原生物学]