毛囊内无色素黑素细胞的超微结构特点  

Ultrastructural Characteristics of Amelanotic Melanocytes from Human Hair Follicles

作　　者：张汝芝[1] 朱文元[2] 夏明玉[3] 王大光[2] 马慧军[2] 

机构地区：[1]安徽蚌埠医学院附属医院皮肤科,233004 [2]南京医科大学第一附属医院皮肤科 [3]南京医科大学电镜室

出　　处：《中华皮肤科杂志》2005年第11期671-673,共3页Chinese Journal of Dermatology

基　　金：国家自然科学基金资助项目(30170861)

摘　　要：目的探讨来自人毛发的毛囊内无色素黑素细胞的超微结构特点。方法胶原酶V消化正常人头皮,获取毛囊。游离毛囊用0．1 mol/L PBS冲洗,胰酶消化,得细胞悬液。将细胞悬液接种到适合黑素细胞生长的培养基。细胞传第3代后,收集细胞,电镜观察摄片。结果透射电镜下可见此细胞为单核圆形或椭圆形细胞,核大,核膜双层,核内常染色体丰富,而异染色质很少。胞质中充满黑素体,多为Ⅱ和Ⅲ期,极少为Ⅰ、Ⅳ期。黑素体内的电子密度颗粒多排列成同心圆形,少数排列呈线状。胞质内高尔基复合体不明显。结论毛囊内无色素黑素细胞超微结构不同于表皮黑素细胞,这种结构变化导致功能上的不成熟,无色素黑素细胞是成熟黑素细胞的贮库。Objective To investigate the uhrastruetural characteristics of amelanotie melanoeytes （AMMCs）. Methods Individual hair follicles from normal human scalp were digested with collagenase type V, then washed in phosphate buffer saline. Hair-follicle cell suspensions were prepared by trypsin and cultured in a medium suitable for melanocyte growth. The keratinocytes were removed by differential trypsinization. Geneticin （100μg/mL） was used to eliminate contaminating fibroblasts. After 3 passages the cells were trypsinized, washed in phosphate buffer saline, and finally processed for transmission electron microscopy. Results Under transmission electron microscope, the cultured cells were round or oval-shaped with a single large nucleus and double-layered karyotheea. Abundant euchromosome but sparse heterochromosome was observed within the nucleus. There were various organelles in the cytoplasm, including mitochondria, rough endoplasmic reticulum （RER）, ribosomes and abundant melanosomes of nearly uniform size. The electronic density granules distributed in a concentric pattern in most of the melanosomes. Golgi complexes were inconspicuous in the cells. Conclusions Compared to epidermal mclanocytes, AMMCs from human hair follicles have different uhrastructural characteristics which implies their functional immaturity. AMMCs may serve as the depot for mature melanocytes.

关 键 词：黑素细胞 黑色素小体 显微镜检查 电子 无色素黑素细胞 细胞超微结构 囊内 黑素细胞生长 圆形细胞 高尔基复合体 表皮黑素细胞 

分 类 号：R751[医药卫生—皮肤病学与性病学]