重组人促红细胞生成素对骨骼肌缺血再灌注损伤的保护作用  

Protective effect of recombinant human erythropoietin on ischemia/reperfusion injury of rat skeletal muscle

作　　者：刘亚[1] 蔡锦方[1] 李振贵 

机构地区：[1]第二军医大学济南临床医学院 [2]陵县中医院外一科

出　　处：《中国矫形外科杂志》2005年第21期1656-1658,共3页Orthopedic Journal of China

摘　　要：[目的]观察重组人促红细胞生成素(rHuEPO)对肢体骨骼肌缺血再灌注(I/R)损伤的保护作用。[方法]建立大鼠后肢缺血再灌注模型。40只大鼠随机均分为:假手术组(Ⅰ组),I/R组(Ⅱ组),I/R+生理盐水组(Ⅲ组),I/R+rHuEPO组(Ⅳ组)。取血浆测定丙二醛(MDA)、肌酸磷酸激酶(CPK)和乳酸脱氢酶(LDH)含量。取骨骼肌标本测定髓过氧化酶(MP0)活性、湿重/干重比(Wet/dry)。[结果]Ⅱ组与Ⅰ组比较,血浆和骨骼肌的各项生化指标显著增高(P<0.01);Ⅳ组血浆及骨骼肌各项测定指标较Ⅱ组相比明显降低(P<0.01)。Ⅲ组和Ⅱ组之间比较,差异无显著意义。[结论]rHuEPO对肢体骨骼肌缺血再灌注损伤有保护作用。[ Objective] To observe the protective effect of recombinant human erythropoietin （rHuEPO） on ischemia/ reperfusion （IR） injury of skeletal muscle in the limbs of rats. [ Method] After establishing the rat model of hind limb ischemia/reperfusion, forty rats were divided into 4 groups ： sham operation group （ Group I ） , 1/R group （ Group Ⅱ ）, I/R ＋ normal saline treatment group （ Group Ⅲ ）, I/R ＋ rHuEPO treatment group （ Group Ⅳ ） . The levels of malodialdehy （MDA） , creatinephosphokinase （CPK） and lactatedehpdrogesase （LDH） in plasma and myeloperoxidase （MPO） ratio, wet/dry of skeletal muscle were measured respectively. [ Result] The indexes of plasma and skeletal muscle in group Ⅱ were significantly higher than those in group I （P 〈 0. 01 ）, while the indexes of the group Ⅳ were significantly lower than those in group Ⅱ （P 〈0. 01 ） , and indexes of group Ⅲ has no difference with those of Group Ⅱ. [ Conclusion] The rHuEPO can protect the skeletal muscle from ischeinia/reperfusion injury in rats.

关 键 词：再灌注损伤 骨骼肌 重组人促红细胞生成素 骨骼肌缺血再灌注损伤 保护作用 丙二醛(MDA) 缺血再灌注模型 血浆测定 肌酸磷酸激酶 生理盐水组 

分 类 号：R685[医药卫生—骨科学]