核因子-_(κ)B与细胞间黏附分子-1在角膜移植排斥反应中的表达及环孢素A对两者表达的影响  

Expressions of nuclear factor-kappa B and intracellular adhesion molecule-1 in corneal graft rejective reaction and the effect of ciclosporin A on their expressions

作　　者：余洪华[1] 陆晓和[1] 张永强[1] 袁伟[1] 彭小娟[1] 姚建兵[1] 

机构地区：[1]南方医科大学珠江医院眼科,广东省广州市510282

出　　处：《中国临床康复》2005年第38期46-49,共4页Chinese Journal of Clinical Rehabilitation

基　　金：广东省自然科学基金项目(032839)~~

摘　　要：目的:探讨核因子-κB在角膜移植排斥反应中的作用,为进一步研究角膜移植排斥反应发病机制及治疗提供新的思路。方法:实验于2004-12/2005-02在南方医科大学珠江医院眼科完成。实验分组:同基因移植组Wistar大鼠5只为供者,Wistar大鼠10只为受者;同种异体移植组Wistar大鼠5只为供者,SD大鼠10只为受者。同种异体移植+环孢素A治疗组Wistar大鼠5只为供者,SD大鼠10只为受者。建立大鼠穿透性角膜移植动物模型,用免疫组织化学染色法检测角膜移植术后植片中核因子-κB和细胞间黏附分子-1表达,显微镜下记数植片中央区400倍视野中阳性细胞数的平均值,并以植片排斥反应指数及病理学表现作参照。结果:纳入实验大鼠30只,均进入结果分析。①同基因移植组角膜植片中的角膜上皮细胞、基质细胞及内皮细胞有核因子-κB和细胞间黏附分子-1的微弱表达,表达强度分别为3.16±1.25,2.83±1.54;同种异体移植组角膜上皮细胞、内皮细胞、基质层细胞及新生血管内皮细胞核因子-κB和细胞间黏附分子-1表达增强,分别为16.77±2.76,13.63±1.93;同种异体移植环孢素A治疗组角膜上皮细胞、内皮细胞、基质层细胞及新生血管内皮细胞有核因子-κB和细胞间黏附分子-1的弱表达,分别为6.77±1.86,4.57±1.91。②各组间核因子-κB和细胞间黏附分子-1的表达强度有显著性差异(P<0.01)。③对比观察发现,各组角膜植片中NF-κB和细胞间黏附分子-1的表达部位和强度相似,相关性分析核因子-κB与细胞间黏附分子-1的表达强度呈正相关(r=0.875,P<0.01),且核因子-κB的表达与排斥反应指数也具有明显的正相关性(r=0.829,P<0.01)。结论:核因子-κB可能通过调控细胞间黏附分子-1等基因的表达,参与角膜移植排斥反应的发生,在角膜移植免疫中起着中心调控者的作用。而免疫抑制剂CsA通过减弱核因子-κB核转位和发挥活性,抑制排�AIM： To investigate the role of nuclear factor-kappa B （NF-κB） in corneal graft rejective reaction, so as to provide new thought for the further research of the pathogenesis and treatment of corneal graft rejective reaction. METHODS： The experiment was carried out in the Department of Ophthalmology, Zhujiang Hospital of Southern Medical University between December 2004 and February 2005. Grouping： homogenic transplantation group （5 Wistar rats as donors and 10 Wistar rats as recipients）, allograft group （5 Wistar rats as donors and 10 SD rats as recipients） and allograft ＋ ciclosperin A treated group （5 Wistar rats as donors and 10 SD rats as recipients）. Rat models of penetrating orthotopical corneal transplantation were established, the expressions of NF-κB and intracellular adhesion molecule-1 （ICAM-1） after the corneal transplantation were detected with the immunohistochemical staining, and the average number of positive cells was enumerated in corneal central zone （×400 micro, field）. The rejection index and pathological manifestations were taken as controls.RESULTS： All the 30 rats were involved in the analysis of results. ① NF-κB and ICAM-1 weakly expressed on the corneal endothelial cells, keratocytes and corneal endothelial cells in the homogemc transplantation group, the expressed intensities were 3.16±1.25 and 2.83±1.54 respectively; NF-κB and ICAM-1 obviously expressed on corneal epithelial cells, keratoeytes, corneal and new vascular endothelial cells in the allograft group, which were 16.77±2.76 and 13.63±1.93 respectively; NF-κB and ICAM-1 expressed weakly on corneal epithelial cells, keratocytes, corneal and new vascular endothelial cells in the allograft ＋ ciclosporin A treated group, which were 6.77±1.86 and 4.57±1.91 respectively. ② The expressed intensities of NF-κB and ICAM-1 were significantly different among the groups （P 〈 0.01）. ③ The comparative observation showed that the expressed site and intensity of NF-κB and ICAM-1 were

关 键 词：NF-κ细胞粘附分子 角膜移植 

分 类 号：R318[医药卫生—生物医学工程]