应用生物力学的方法观察十一酸睾酮对老年雄性大鼠骨的作用  

Effects of testosterone undecanoate on bone in elder male rats observed with biomechanics approach

作　　者：李孝鹏[1] 符诗聪[2] 张风华[2] 朱雅萍[2] 

机构地区：[1]中华人民共和国司法部司法鉴定科学技术研究所,上海市200063 [2]上海市伤骨科研究所,上海市200025

出　　处：《中国临床康复》2005年第38期50-52,共3页Chinese Journal of Clinical Rehabilitation

基　　金：上海教委资助(03BK26)~~

摘　　要：目的:运用生物力学的方法,观察雄激素十一酸睾酮在动物大体内对老年大鼠骨的作用。方法:实验于2002-10在上海市伤骨科研究所完成。鼠龄23个月Wistar雄性大鼠20只,随机分为治疗组10只、空白对照组10只,另选鼠龄3个月Wistar雄性大鼠10只为青年对照组,治疗组十一酸睾酮26.7mg/kg灌胃,1次/d给药两周,后改为维持剂量(药量减半)用药,空白对照组和青年对照组给予普通饮食。1个月后取材,并在取材前第3天、第13天行四环素标记。①取各组大鼠左侧股骨做三点抗弯实验测定最大载荷、最大应力、最大应变、弹性模量、抗弯硬度、吸收能量。②取各组大鼠第五腰椎做椎体抗压实验测定最大载荷、最大应力、最大应变、弹性模量、抗压硬度、吸收能量。结果:实验大鼠30只均进入结果分析。①与空白对照组相比,股骨的最大载荷、吸收能量、最大应力值显著增高[治疗组:(129.08±12.36)N,(49.40±7.4)Nmm,(128.47±16.73)N/mm2,青年对照组:(132.04±25.84)N,(64.27±10.73)Nmm,(145.78±23.54)N/mm2,空白对照组:(102.03±11.62)N,(32.00±8.50)Nmm,(107.55±17.37)N/mm2,P<0.01],弹性模量、抗弯硬度值亦明显增加[治疗组:(4072.1±434)mN,(214.73±40)N/mm,青年对照组:(4318.6±201)mN,(281.17±66.85)N/mm,空白对照组:(3624.7±269)mN,(189.47±45.2)N/mm,P<0.05],其中最大载荷增加26.5%,抗弯硬度增加13.3%。②与空白对照组相比,治疗组第五椎体最大载荷增加29.2%(P<0.05),抗压硬度增加34.6%(P<0.01),弹性模量值增加39.93%(P<0.01),最大应力值增加19.45%(P<0.05)。结论:雄激素能够提高骨的力学性能,增加骨强度。AIM： To observe the effects of androgen testosterone undecanoate on bone in elder male rats with biomechanics method. METHODS： The experiment was conducted at the Shanghai Municipal Institute of Orthopedics and Trauma during October 2002. Twenty male Wistar rats, twenty-three months old, were randomly divided into two groups： therapy group and non-therapy control group with 10 in each group. Simultaneously, ten male Wistar rats （three months old） were selected as youth control group. The rats in the therapy group were treated with gastric perfusion with 26.7 mg/kg testosterone undecanoate, once a day for two weeks, and then changed to maintain dosage （the dosage reduced a halt）. Those in the non-therapy control group and the youth control group were given common food. The materials were drawn after one month, and all rats were subjected to tetracycline labeling at the 34, the 134 days before drawing. ①The left femurs were gained from the rats in every group to assess the maximum load, maximum stress, maximum strain, elastic mould, bending resistance consistency and absorption capacity with threepoint bending resistance test. ② L-5 vertebras were gained from the rats in every group to detect maximum load, maximum stress, maximum strain, elastic mould, press resistance consistency and absorption capacity with vertebral compression resistance test. RESULTS： Totally 30 experimental rats were involved in the result analysis. ① Compared with the non-therapy control group, the values of femoral maximum load, absorption capacity, maximum stress all significantly enhanced [therapy group： （129.08±12.36）N, （49.40±7.4）Nmm, （ 128.47±16.73 ）N/mm^2, youth control group： （ 132.04±25.84 ）N, （64.27±10.73）Nmm, （145.78±23.54）N/mm^2, non-therapy control group： （102.03±11.62）N, （32.00±8.50）Nmm, （107.55±17.37）N/mm^2,p 〈 0.01]. The values of elastic mould and bending resistance consistency increased obviously [therapy group： （4 072.1±434） mN, （214.73±40

关 键 词：骨 生物力学 雄激素类 大鼠 

分 类 号：R496[医药卫生—康复医学]