转化生长因子β对兔尺骨骨折愈合后生物力学性能的影响  

Effects of transforming growth factor-beta on biomechanical property of fracture healing in ulna fracture rabbits

作　　者：卢卫忠[1] 唐康来[2] 赵麟丰[1] 杨柳[2] 何静 周琦[1] 王小波[1] 许建中[2] 

机构地区：[1]重庆市涪陵中心医院骨科,重庆市408000 [2]解放军第三军医大学西南医院骨科,重庆市400038 [3]重庆市药剂学校,重庆市400032

出　　处：《中国临床康复》2005年第38期95-97,共3页Chinese Journal of Clinical Rehabilitation

基　　金：重庆市科委应用基础研究基金资助(2000177)~~

摘　　要：目的:分析外源性转化生长因子β对兔尺骨骨折愈合后整体骨力学强度和密质骨力学强度的影响。方法:实验于2001-12/2002-06在第三军医大学实验动物中心完成。采用兔尺骨骨折模型,36只成年兔随机分组,9只/组。根据每天注射转化生长因子β剂量不同分为0μg(对照组),1μg,10μg,20μg组;术后第3天开始在转化生长因子β各治疗组右侧尺骨中段尺侧骨折区注射转化生长因子β溶液100μL(分别含转化生长因子β1μg,10μg,20μg),在对照组相应部位注射磷酸盐缓冲液100μL,1次/d,共10次。8周后采集骨标本,通过三点弯曲法测骨折愈合后整体骨弯曲结构力学性能的变化,取断端密质骨,用三点弯曲、压缩和拉伸方法测骨试件的材料力学特性的变化,同时测骨痂几何参数和骨密度的变化来对骨折愈合进行评估。结果:36只白兔均完成全部实验,无死亡。全部纳入分析。①在骨折8周后,各转化生长因子β治疗组(1μg、10μg、20μg组)尺骨骨痂中份的冠状径、矢状径和骨痂厚度明显高于对照组,而转化生长因子β各治疗组之间及其与对照组之间的骨密度均无明显差别。②转化生长因子β各治疗组的破坏载荷、极限强度和弹性模量明显高于对照组,在转化生长因子β1～10μg/d范围内,随剂量增加而增大,到20μg/d时,增大已不明显。③各转化生长因子β治疗组尺骨弯曲、压缩和拉伸的极限强度明显高于对照组,弯曲和压缩的弹性模量也明显高于对照组,在转化生长因子β1～10μg/d范围内,随治疗剂量增加而增加,到20μg/d时,增加已不明显。结论:局部应用外源性转化生长因子β可促进骨痂形成,增强骨折愈合后整体骨弯曲的破坏载荷、骨试件的弯曲、压缩和拉伸的极限强度以及弯曲和压缩的弹性模量,并呈一定的剂量依赖关系,以局部注射转化生长因子β10μg/d的作用最明显。AIM： To analyze the effects of exogenous transforming growth factor-beta （TGF-β） on mechanics intensity of integral, bone and compact bone alter fracture healing of ulna in rabbits. METHODS： The experiment was conducted at the experimental animal center of the Third Military Medical University from December 2001 to june 2002. Using fracture of ulna of rabbits, 36 adult rabbits were assigned randomly, 9 rabbits in each group. According to the different dosage of injecting TGF-β every day, they were divided into 0 μg （control group）, 1 μg, 10 μg and 20 μg group, At the 3^rd day after operation, they were treated with 100 μL TGF-β solution at the middle ulnaris fracture region of ulnar at right side in the every TGF-β treatment group （including 1 μg, 10 μg and 20 μg TGF-β, respectively）. Those in the control group were injected with 100 μL phosptat buffe at the same part as above-mentioned, once a day for 10 times. Eight weeks later, the bone samples were gained. Meehanie properties changes of integral bone bending structure after fracture healing was measured with three-point bending method. The changes of material mechanical properties of these bones were detected with three-point bending, compressing and expending method. Meanwhile, the changes of geometrie parameters of callus and bone mineral density （BMD） were detected to evaluate the fracture healing. RESULTS： All of 36 rabbits were performed the experiment, without death, and involved in the result analysis. ①Eight weeks later of fracture, the coronal inside diameter, arrow shape inside diameter and callus depth in callus of ulnar in every TGF-β treatment group （ 1 μg, 10 μg and 20 μg group）were higher significantly as compared with the control group, while the BMD in every TGF-β treatment groups and in control groups had insignificant difference. ② The broken load, the ultimate bending strength, the bending elastic modulus in every TGF-β treatment group were significantly higher than those in the control gro

关 键 词：生物力学 转化生长因子Β 骨折 

分 类 号：R681[医药卫生—骨科学]