日本绒螯蟹ND2基因全序列测定与分析  

The complete sequence of the mitochondrial ND2 gene of Eriocheir japonica

作　　者：柳广东[1] 高天翔[1] 刘进贤[1] 渡边精一 

机构地区：[1]中国海洋大学海水养殖教育部重点实验室,山东青岛266003 [2]东京水产大学水生生物系

出　　处：《水产学报》2005年第5期711-714,共4页Journal of Fisheries of China

基　　金：中国海洋大学海水养殖教育部重点实验室开放课题(200411)

摘　　要：Degenerate primers were designed according to the conserved sequences of NADH dehydrogenase subunit 2（ND2） gene in mitochondrial tRNAMet and tRNATrp gene respectively,and the complete sequence of the ND2 gene of Eriocheir japonica was determined.The results indicated that the length of ND2 gene of Eriocheir japonica was 1009 bp.The A,T,G,C contents of the gene were 28.54%（288 bp）,44.20%（446 bp）,8.23%（83 bp）,and 19.03%（192 bp） respectively;and the sequence was AT rich and biased.The start and stop codons were ATC and A respectively.The homology analysis of nucleotide sequence showed that the similarities in ND2 gene between Eriocheir japonica and other crustacean were comparatively low,which suggested that ND2 gene could be effective molecular marker for further study on phylogeny of Eriocheir species.Degenerate primers were designed according to the conserved sequences of NADH dehydrogenase subunit 2 （ND2） gene in mitochondrial tRNAMet and tRNAvrp gene respectively, and the complete sequence of the ND2 gene of Eriocheir japonica was determined. The results indicated that the length of ND2 gene of Eriocheir japonica was 1009 bp. The A, T, G, C contents of the gene were 28.54% （288 bp）, 44.20% （446 bp）, 8.23% （83 bp）, and 19.03% （ 192 bp） respectively; and the sequence was AT rich and biased. The start and stop codons were ATC and A respectively. The homology analysis of nucleotide sequence showed that the similarities in NEE gene between Eriocheir japonica and other crustacean were comparatively low, which suggested that ND2 gene could be effective molecular marker for further study on phylogeny of Eriocheir species.

关 键 词：日本绒螯蟹 ND2基因 全序列 

分 类 号：S917[农业科学—水产科学]