三种特异性血清学方法诊断流行性出血热的评价  

EVALUATION OF THREE SPECIFIC SEROLOGICAL METHODSIN THE DIAGNOSIS OF EPIDEMIC HEMORRHAGIC FEVER

作　　者：张润苍 唐利平[1] 王世伟[1] 李明禄[1] 周小梅[1] 蒙利民[1] 雷强[1] 贺昌明[2] 沈光辉[2] 

机构地区：[1]重庆市第二人民医院传染科 [2]重庆市第二人民医院中心实验室

出　　处：《重庆医药》1989年第5期7-10,共4页

摘　　要：本文用IFAT、ELISA、HI平行检测了出血热患者血清161份的出血热病毒抗体，阳性57.9～95.1％；ELISA、HI和IFAT分别于2、4病日出现阳性；ELISA、IFAT分别于60、90病日阴转，HI94病日仍阳性；HI的阳性率（95.1％）高于IFAT（IgM57.9％、IgG88.4％）及ELISA（IgM75.6％、IgG65.9％），其发热期阳性率亦高于其他两法；ELISA-IgM、IgG的阳性率及ELISA-IgM、HI抗体的滴度随病情加重而增加。因此，ELISA（主要是ELISA-IgM）和HI具有高度特异性、敏感度，阳性出现早，HI在三法中灵敏度最高。The antibodies against epidemic hemorrhagic fever (EHF) virus of 164 serum samples from patients with EHF, 50 samples from normal subjects and 50 samples from patients other than EHF were determined in parallel by IFAT.ELISA and HI. There-suits showed that positive rates were 57.9?5.1% in the patients with EHF. ELISA, HI and IFAT changed into positive from 2nd to 4th day. ELISA and IF AT turned re-spectively to negative in 60 and 90 days,HI-antibody was also positive in 94 days. The positive rate (95,1%) of HI was higher than IFAT (IgM 57.9%, IgG 88.4%) and ELISA(IgM75.6%,IgG 65.9%)(P<0.05).In febrile phase,the positive rate of HI(95.5%) was higher than the other two methods (P<0.05). The positive rates of ELISA-IgM, IgG became higher and titers of ELISA-IgM, Hi-antibody tended to elevating with severity of the disease. Thus, ELISA (chief ELISA-IgM) and HI are highly specific, sensitive and appear early in the disease.

关 键 词：流行性出血热 IFAT ELISA HI 诊断 

分 类 号：R512.804[医药卫生—内科学]