辛伐他汀对糖尿病大鼠肾脏TGF-β系统的影响  

Effects of simvastatin on TGF-β system of diabetic rat kidneys

作　　者：陈卓雄[1] 雷闽湘[1] 朱丽芳[1] 张军[1] 

机构地区：[1]中南大学湘雅医院内分泌科,长沙410008

出　　处：《中南大学学报（医学版）》2005年第5期593-596,共4页Journal of Central South University ：Medical Science

基　　金：湖南省卫生厅科研基金(2003-824)

摘　　要：目的:探讨辛伐他汀对糖尿病大鼠肾脏转化生长因子β1(TGF-β1)和转化生长因子βⅡ型受体(TβⅡR)表达的影响及其肾脏的保护机制。方法:SD大鼠单次腹腔注射55mg/kg链脲佐菌素(STZ)制备糖尿病大鼠模型。糖尿病大鼠随机分成糖尿病组、辛伐他汀治疗组[4mg/(kg·d)]和正常对照组。用免疫组织化学,RT-PCR和Western印迹法检测16周大鼠肾脏TGF-β1和TβⅡR表达的变化。结果:与正常对照组比较,糖尿病组和辛伐他汀治疗组TGF-β1和TβⅡR表达均增加(P<0.05)。治疗组TGF-β1和TβⅡR表达显著低于糖尿病组(P<0.05)。结论:辛伐他汀可以通过下调TGF-β1和TβⅡR的表达,抑制TGF-β系统信号通路的过度激活,减少糖尿病大鼠尿蛋白,延缓肾脏病变的进展。Objective To investigate the effects and mechanism of renal benefit of simvastatin on diabetic rat kidneys. Methods Twenty STZ-indueed SD rats and 10 normal rats were assigned to diabetic rat （DM） group, simvastatin [4 mg/（ kg · d） 1 treatment （S） group and normal control （C） group. Immunohistochemistry, RT-PCR and western-blot were employed to examine the changes of the mRNA and protein expression of TGF-β1 and Tβ Ⅱ R in the kidneys of the rats. Results Compared with the normal control group, both the mRNA and protein expression of TGF-β1 and Tβ Ⅱ R in the diabetic rat group and treatment group were significantly increased （ P 〈 0.05 ）. Compared with the diabetic rat group, simvastatin eould markedly decrease the mRNA and protein expression of TGF-β1 and Tβ Ⅱ R （P 〈0.05）. Conclusion Simvastatim may play a protective role in the diabetic kidneys by down-regulating TGF-β1 and Tβ Ⅱ R and inhibiting the TGF-β signal pathway.

关 键 词：糖尿病 转化生长因子Β 辛伐他汀 

分 类 号：R587.1[医药卫生—内分泌]