脊髓前体细胞移植在大鼠臂丛神经根性撕脱伤中的应用研究  

STUDY ON THE EFFECT OF SPINAL NEURAL PROGENITOR TRANSPLANTATION ON TREATING BRACHIAL PLEXUS AVULSED INJURY

作　　者：张军[1] 张信英[1] 曹阳[1] 付春江[1] 

机构地区：[1]哈尔滨医科大学附属第一医院骨科,哈尔滨150001

出　　处：《中国修复重建外科杂志》2005年第11期864-867,共4页Chinese Journal of Reparative and Reconstructive Surgery

基　　金：黑龙江省十五攻关课题资助项目(G99C19-10-1)~~

摘　　要：目的观察脊髓前体细胞移植对臂丛神经根性撕脱伤的疗效。方法将54只大鼠制作左侧臂丛神经根性撕脱伤,行撕脱神经根移植术动物模型,分为新鲜组、陈旧组及对照组,每组18只。培养脊髓神经前体细胞,并进行传代和分化,鉴定、标记后分别于模型制作1周(新鲜组15只)和2个月(陈旧组14只)以1×105/μl脊髓前体细胞植入存活动物模型体内,对照组(12只)不植入细胞。细胞移植后2个月行大鼠运动功能恢复评定,观察电生理变化,并行免疫组织化学观察移植细胞。结果获取的异体脊髓前体细胞能增生为神经球,分化为神经元及神经胶质细胞。新鲜组大鼠14只运动功能恢复,其中2只5分,2只4分,6只3分,4只2分,1只无恢复;陈旧组7只功能恢复,1只3分,2只2分,4只1分,7只无恢复;对照组5只功能恢复,1只3分,1只2分,3只1分,7只无恢复。且功能恢复良好的大鼠神经电生理也恢复。免疫组织化学观察:新鲜组移植细胞分化成有较长突触的运动神经元;陈旧组移植细胞成团存在,分化不良。结论臂丛神经根性撕脱伤行撕脱神经根移植术后,早期移植1×105/μl脊髓前体细胞具有促进其恢复作用。Objective To explore the effect of spinal neural progenitor transplantation to the cervical spinal on treating brachial plexus injury with the reimplantation of the avulsed spinal roots. Methods The brachial plexus avulsed injury model was made on 54 rats and they were evenly divided into 3 groups： fresh group, chronic group, control group. The spinal neural progenitor was cultured and identified. Then 10μl（1×10^5/μl）cells were labelled with BrdU and transplanted into the fresh group （15 rats survived, being model for 1 week） and the chronic group （14 rats survived, being model for 2 months）. No cell was transplanted into the control group. Two months after the transplantation, the recovery of function of the injured limb was evaluated. Electrophysiologic study and immunohistochemical study of the injured limb were made. Results Spinal neural progenitors were isolated from the spine and became neural sphere. The neural spheres were differentiated into neurons and astrocytes. Fourteen rats out of 15 in the fresh group were recovered, 7 rats out of 14 in the chronic group were recovered, and 5 rats out of 12 in the control group were recovered. Immunohistochemical study indicated that the transplanted progenitors in fresh group survived and differentiated into the neural cells, and the transplanted progenitors in chronic group existed and did not differentiate well. Conclusion Transplanted spinal neural progenitors can promote the recovery of the brachial plexus injury with the reimplantation of the avulsed spinal root.

关 键 词：臂丛神经根性撕脱伤 神经根再植 脊髓神经前体细胞移植 大鼠 

分 类 号：R651.3[医药卫生—外科学] R622.3[医药卫生—临床医学]