血管内皮细胞(ECV304)表达的HLA-G1对NK细胞杀伤活性的抑制作用  

HLA-G1 molecule expressed by ECV304 cells inhibit cytotoxic activity of allogeneic NK cells

作　　者：张彩娥[1] 韩军艳[1] 杨惠军[1] 吴雄文[1] 黄亚非[1] 梁智辉[1] 龚非力[1] 

机构地区：[1]华中科技大学同济医学院免疫学系,湖北武汉430030

出　　处：《细胞与分子免疫学杂志》2005年第6期676-678,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目(No.30271201)

摘　　要：目的:证实HLA-G1分子能够抑制NK细胞对同种血管内皮细胞系的杀伤作用。方法:采用脂质体介导的DNA转染技术,以构建的真核表达质粒pcDNA3-HLA-G1转染人脐静脉内皮细胞系ECV304,再用免疫荧光法检测表达的HLA-G1分子。并用MTT比色法检测HLA-G1对NK细胞杀伤活性的影响。结果:ECV304细胞上可稳定表达HLA-G1。NK细胞对空质粒pcDNA3转染的ECV304细胞的杀伤率为(50.6±18.1)%;而对pcDNA3-HLA-G1转染的ECV304细胞的杀伤率为(29.7±11.4)%,两者差异具有显著意义(P<0.01)。结论:HLA-G1分子可明显抑制NK细胞对同种血管内皮细胞的杀伤效应。AIM： To study the effect of HLA-GI molecule expressed by an endothelial cell line （ECV304） on the cytotoxic activity of allogeneic NK cells. METHODS： ECV304 cells were transfected with recombinant plasmid pcDNA3-HLA-G1 by the liposome transfection, and the expressed HLA-G1 on the cell surface was detected by indirect immunofluorescent assay and flow cytometry. The cytotoxic activity of allogeneic NK cells against ECV304 cells was analyzed by the MTT method. RESULTS： HLA-G1 was expressed on the surface of the transfected ECV304 cells. The specific lysis of NK cells against plasmid pcDNA3 transfected ECV304 was （50.6 ± 18. 1 ）%, while the specific lysis against pcDNA3-HLA-G1 transfected ECV304 was （29. 7±11. 4） %, which was significantly lower than the former （ P 〈 0. 001 ）. CONCLUSION： HLA-G1expressed by the ECV304 cells can inhibit cytotoxicity of allogeneic NK cells.

关 键 词：HLA—G1 ECV304 NK细胞 杀伤抑制 

分 类 号：R392.12[医药卫生—免疫学]