TNF-α含量的改变与肾小管细胞缺氧-复氧性损伤关系的研究  

The relationship between TNF-α and the injury of renal tubular cells caused by anoxia/reoxygenation

作　　者：董文斌[1] 杜逸亭[1] 陈跃[2] 航永伦[3] 陈枫[4] 邓存良[4] 

机构地区：[1]泸州医学院附属医院新生儿科,四川泸州646000 [2]泸州医学院附属医院核医学科,四川泸州646000 [3]泸州医学院附属医院检验科,四川泸州646000 [4]泸州医学院附属医院传染与免疫研究室,四川泸州646000

出　　处：《细胞与分子免疫学杂志》2005年第6期690-692,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：四川省杰出青年学科带头人培养基金资助(No.04ZQ026-033);四川省中医管理局重点科研基金资助(No.200239)

摘　　要：目的:探讨TNF-α含量的改变与肾小管上皮细胞株(HK-2)缺氧-复氧性损伤的关系。方法:以HK-2细胞株作为研究材料,采用液体石蜡覆盖法建立缺氧损伤模型,分别在缺氧4、12、24h和复氧4、12、24h,采用放射免疫分析法(RIA)、生化法和台盼蓝拒染法,检测培养液中TNF-α的含量、乳酸脱氢酶(LDH)的活性、细胞计数及活细胞率的改变。结果:缺氧后4h,TNF-α的含量、LDH的活性开始升高,细胞总数及活细胞计数开始下降,并于缺氧后24h达高峰。TNF-α含量的改变与LDH活性的升高呈显著的正相关(r=0.89,P<0.05),与活细胞计数的下降呈显著的负相关(r=-0.91,P<0.05)。结论:肾小管缺氧-复氧后,TNF-α的产生增多,并参与了肾小管细胞损伤的过程。AIM： To investigate the relationship between TNF-α and renal tubular cell injury caused by anoxia/ reoxygenation. METHODS： Human renal proximal tubular cell line HK-2 was used as model. Anoxia/reoxygenation were produced by covering/de-covering the cell culture with liquid paraffin wax. The level of TNF-α and the activity of lactate dehydrogenase （LDH） in the culture medium was determined by radioimmunoassay （RIA） and biochemical methods, respectively. Trypan blue exclusion was used to measure cell viability. RESULTS： Anoxia/reoxygenation could increase TNF-α level and LDH activity, but decrease viability of HK-2 cells. TNF-α level was positively correlated with LDH activity （ r = 0.89, P 〈 0.05 ） and negatively with the cell viability （ r = - 0.91, P 〈 0.05 ）. CONCLUSION： TNF-α induced by anoxia/reoxygenation may participate in the process of renal tubular cell injury.

关 键 词：缺氧/复氧 TNF-Α 肾小管上皮细胞 

分 类 号：R392.11[医药卫生—免疫学]