白藜芦醇对小鼠T细胞活化、增殖及细胞因子分泌的影响  

The effects of resveratrol on the activation,proliferation and cytokine expression of murine T lymphocytes

作　　者：俞瑜[1] 曾耀英[1] 季煜华[1] 肇静娴[1] 刘良[2] 

机构地区：[1]暨南大学组织移植与免疫教育部重点实验室,广东广州510632 [2]香港浸会大学中医药学院

出　　处：《细胞与分子免疫学杂志》2005年第6期697-699,703,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：香港特区政府大学教育资助委员会基金资助(No.2144/03M);广东省自然科学基金项目资助(No.04300653)

摘　　要：目的:研究白藜芦醇(RSV)对小鼠T淋巴细胞增殖及细胞因子分泌的影响,为阐明其免疫抑制作用提供实验依据。方法:无菌分离小鼠淋巴结细胞,加入不同浓度的RSV预先孵育1h,用多克隆刺激剂佛波醇酯和离子霉素刺激T细胞活化增殖。采用3H-TdR掺入法检测RSV对T细胞增殖的影响;用RT-PCR检测IL-2及IFN-γmRNA的表达;用胞内细胞因子染色法分析IL-2和IFN-γ的分泌。结果:RSV能抑制T细胞增殖,也能在mRNA水平及蛋白水平上抑制IL-2及IFN-γ的分泌。结论:RSV对T细胞的免疫抑制作用,可能与其抑制T细胞的活化、增殖和细胞因子的分泌有关。AIM： To study the effects of resveratrol （RSV） on the activation, proliferation and cytokine expression of murine T lymphocytes. METHODS： Murine T lymphocytes were isolated from lymph nodes and cultured in vitro. The lymphocytes were pre-treated with RSV for I h prior to activation with PDB plus ionomycin. 3H-TdR incorpo- ration was used to detect T cell proliferation. IL-2 and IFN-γ mRNA were detected by RT-PCR, IL-2 and IFN-γ proteins were detected by intracellular cytokine staining with flow cytometry. RESULTS： RSV can inhibit the proliferation and IL- 2 and IFN-γ proteins expression of T lymphocytes. CON- CLUSION： The immunosuppressive action of RSV may be related with its inhibitory effects on T cell activation, proliferation and cytokine expression.

关 键 词：白藜芦醇 活化 增殖 细胞因子 

分 类 号：R392.12[医药卫生—免疫学]