海南岛长蒴母草和金腰箭上存在粉虱传双生病毒的侵染  

Detection of Whitefly-transmitted Geminivirus in Lindernia anagallis and Synedrella nodiflora in Hainan Island China

作　　者：车海彦[1] 罗大全[1] 杨旭光[1] 叶莎冰[1] 

机构地区：[1]中国热带农业科学院环境与植物保护研究所,海南儋州571737

出　　处：《热带作物学报》2005年第2期81-83,共3页Chinese Journal of Tropical Crops

基　　金：中国热带农业科学院科技基金资助项目

摘　　要：从海南儋州的长蒴母草(Linderniaanagallis)上采集了呈现叶脉黄化、叶背叶脉突起的3个病样,从金腰箭(Synedrellanodiflora)上采集到叶片不均匀褪绿、叶背叶脉突起的3个病样。利用根据粉虱传双生病毒基因组DNA基因间隔区及外壳蛋白基因保守序列设计的简并引物,对这6个样品进行了PCR检测,结果发现所有样品均能扩增到预期大小的DNA片段。PCR产物经克隆后进行序列测定,BLAST结果表明,该序列为双生病毒基因组DNA序列,该2种杂草上均存在双生病毒的侵染。Three virus-infected Lindernia anagcdlis samples showing yellow vein, vein plum symptom and three virus-infected Synedrella nodiflora samples showing mosaic, vein plum symptom were collected in Danzhou, Hainan Province. A degenerate primer pair was designed to the conserved sequence of CP gene and intergenic spacer of WTG. A single PCR product of expected size was amplified from six samples by PCR, cloned and sequenced. That PCR product had a geminiviral gene origin was demonstrated by BLAST. All samples were infected with geminiviruses.

关 键 词：粉虱传双生病毒 长蒴母草 金腰箭 PCR 

分 类 号：S432.41[农业科学—植物病理学]