维甲酸致胚胎神经管畸形过程中RARα和RARβ表达水平变化的实验研究  

Experimental study on the changes of expressions of RARα and RARβ during neural tube defects induced by retinoic acid

作　　者：芦志红[1] 刘凯[1] 高英茂[1] 

机构地区：[1]山东大学医学院组织学与胚胎学教研室,山东济南250012

出　　处：《山东大学学报（医学版）》2005年第9期785-787,共3页Journal of Shandong University:Health Sciences

基　　金：山东省自然科学基金资助课题(Y99C01)

摘　　要：目的:探讨过量维甲酸(RA)对金黄地鼠胚胎神经管维甲酸受体α(RARα)和维甲酸受体β(RARβ)蛋白表达的影响。方法:采用免疫组织化学方法及图像分析,半定量分析金黄地鼠神经管正常发生及RA致神经管畸形过程中RARα和RARβ表达水平的变化。结果:过量RA可使RARα和RARβ在金黄地鼠神经管中的表达水平呈现短时下降,而后又大幅上升的变化。结论:过量RA引起的RARα和RARβ表达水平的变化与RA致金黄地鼠神经管畸形相关。Objective： To investigate the effect of excess retinoic acid （RA） on the expressions of RARα and RARβ in neural tube of golden hamsters. Methods： Through immunohistochemistry and image analysis methods, the difference of expressions of RARα and RARβ between normal and defected neural tube induced by excess RA was analyzed in golden hamsters. Results： Excess RA made the expressions of RARα and RARβ decline firstly, then increase significantly in neural tube of golden hamster. Conclusion： The expressions of RARα and RARβ induced by excess RA are related to the neural tube defects in golden hamster.

关 键 词：受体 维甲酸 神经管畸形 金黄地鼠 

分 类 号：R321.6[医药卫生—人体解剖和组织胚胎学] R-332[医药卫生—基础医学]