不同年龄大鼠坐骨神经损伤后睫状神经营养因子对脊髓组织中天冬氨酸特异酶切的半胱氨酸蛋白酶3表达的影响(英文)  

Influence of ciliary neurotrophic factor on spinal cord caspase-3 expression in rats of different age following sciatic nerve damage

作　　者：李陶[1] 王禾[2] 卓豫[2] 

机构地区：[1]解放军第三军医大学野战外科研究所大坪医院超声科,重庆市400042 [2]解放军第三军医大学野战外科研究所大坪医院第五研究室,重庆市400042

出　　处：《中国临床康复》2005年第37期166-169,共4页Chinese Journal of Clinical Rehabilitation

基　　金："九七三"国家重点基础研究发展规划项目(G1999054206)~~

摘　　要：背景:天冬氨酸特异酶切的半胱氨酸蛋白酶3是一种促进细胞凋亡的半胱氨酸蛋白酶,睫状神经营养因子具有多种生物活性,能保护损伤后多种神经元特别是运动神经元,减少神经细胞损伤的发生。目的:观察睫状神经营养因子对天冬氨酸特异酶切的半胱氨酸蛋白酶3在坐骨神经损伤后不同年龄大鼠脊髓组织中的表达以及在不同年龄阶段的变化规律和调控特点。设计:随机对照动物实验。单位:解放军第三军医大学野战外科研究所大坪医院超声科、第五研究室。材料:实验于2000-04/2001-12在解放军第三军医大学野战外科研究所完成。Wistar大鼠,体质量30～100g的幼年大鼠(鼠龄20d)、200～350g的成年大鼠(鼠龄4个月)、400～800g的老年大鼠(鼠龄18～24个月)各270只,雌雄不限,共810只。方法:①实验动物分组:各年龄组大鼠随机分为正常组(n=18)、睫状神经营养因子组(n=126)和生理盐水组(n=126),睫状神经营养因子组和生理盐水组分为术后1d、3d、1周、2周、4周、8周、12周组。②动物模型制作:睫状神经营养因子组和生理盐水组切除2mm右侧坐骨神经,用硅胶管连接近、远侧神经残端制作神经再生小室,睫状神经营养因子组再生小室内注入25mg/L重组睫状神经营养因子15μL,生理盐水组再生小室内注入生理盐水15μL。③待测标本的制作及检测:分别取各组动物6只,取脊髓L3～5节段,进行天冬氨酸特异酶切的半胱氨酸蛋白酶3免疫组织化学检测、天冬氨酸特异酶切的半胱氨酸蛋白酶3活性检测和Westernblotting检测。主要观察指标:①免疫组织化学检测的各组大鼠脊髓天冬氨酸特异酶切的半胱氨酸蛋白酶3的表达。②Westernblotting检测脊髓天冬氨酸特异酶切的半胱氨酸蛋白酶3的分布与表达强度变化。③天冬氨酸特异酶切的半胱氨酸蛋白酶3活性变化。结果:810只大鼠进入结果分析。①免疫组织化学检�BACKGROUND： Caspase-3 is a cysteine proteinase that can promote cell apoptosis. Ciliary neurotrophie factor has many kinds of biological activities, such as protecting various neurons from injury, especially motorial neurons, thereby reducing the occurrence of nerve cell injury. OBJECTIVE： To observe the Influence of ciliary neurotrophie factor on spinal cord caspase-3 expression in different ages rats following sciatic nerve injury, aiming to make further investigation about the changing regularity and modulating character of peripheral nerve damage at various ages rats. DESIGN： Randomized controlled experiment. SETTING： Ultrasound Department and the 5^th Research Institute of Daping Hospital, Field Surgery Research Institute of the 3^rd Military Medical University of Chinese PLA. MATERIALS： This experiment was carried out at the Field Surgery Re- search Institute of the 3^rd Military Medical University of Chinese PLA from April 2000 to December 2001. Altogether 810 wistar rats including infant rats with body mass of 30-100 g （birth age of 20 days）, adult rats of 200- 350 g （birth age of 4 m）, elder rats of 400-800 g （birth age of 18-24 m）, were selected with 270 rats in each age stage. Female and male does not limit. METHODS： ① Experimental animal grouping： Various age rats were randomly dividod into normal group （n=18）, ciliary neurotrophic factor group （n=126） and physiological saline group （n=126）, rats in ciliary neurotrophic factor group and physiological saline group were subdivided into 1 day, 3 days, 1 week, 2 weeks, 4 weeks, 8 weeks, 12 weeks subgroups. ② Animal model preparation： In Ciliary neurotrophic factor group and physiological saline group, rats were cut off a piece of sciatic nerve of 2 mm long, both ends connected with silica tube for constructing neuranagenesis cab, in which 15μL recombined ciliary neurotrophic factor （25 mg/L） was injected in ciliary neurotrophic factor group, and 15μL physiological saline in physiological saline group. ③

关 键 词：坐骨神经/损伤 半胱氨酸天冬氨酸蛋白酶 神经组织蛋白质类 大鼠 年龄 

分 类 号：R651.3[医药卫生—外科学]