杂色鲍碱性磷酸酶在醇、醛和酮溶液中的失活作用  

Inactivation effect of alkaline phosphatase from Haliotis diversicolor in the solutions of alcohol, aldehyde and ketone

作　　者：陈巧[1,2] 洪燕飞[1] 廖金花[1] 庄总来[1] 陈清西[1] 

机构地区：[1]细胞生物学与肿瘤细胞工程教育部重点实验室,厦门大学生命科学学院生物化学与生物技术学系,福建厦门361005 [2]福建教育学院生化系

出　　处：《台湾海峡》2005年第4期469-473,共5页Journal of Oceanography In Taiwan Strait

基　　金：教育部跨世纪人才经费和回国启动经费资助项目

摘　　要：报道了杂色鲍碱性磷酸酶(ALP)在甲醇、乙醇、丙醇、甲醛、丙酮等溶液中的失 活动力学．结果表明:酶的剩余活力随着有机溶剂浓度的增大而迅速下降．测得上述 5种有机溶剂对该酶的半抑制率(IC50)分别为5．41、3．07、1．80、31．0×10-3、2．30 mol／dm3．在这些有机溶剂溶液中酶的失活过程都是可逆反应．检测醇、醛、酮对该酶 的失活作用机理,结果表明甲醇、乙醇、丙醇对杂色鲍ALP的失活作用均为非竞争性 机制,其抑制常数分别为5．36、3．02、1．80mol／dm3．甲醛、丙酮对杂色鲍ALP的失活 作用都呈现为混合型抑制,其对游离酶的抑制常数(K1)分别为24．7×10-3、1．66 mol／dm3;对酶-底物络合物的抑制常数(KIS)分别为56．2×10-3、5．40mol／dm3．The inactivation effects of alkaline phosphatase （ALP） from Haliotis diversicolor in organic solvents （methanol, ethanol, propanol , aldehyde, acetone）were studied using the kinetic method of Lineweaver-Burk plot. And their inhibition types and inhibition constants were determined. Results showed that the enzyme activity rapidly declined with increasing organic solvent concentrations. The IC50, the concentrations leading to 50% activity lost, of methanol, ethanol, propanol, aldehyde, and acetone were estimated as 5.41,3.07, 1.80,31.0×10^-3,2.30mol/dm^3 respectively. The inactivation of the enzyme in these organic solvents was reversible reaction. The inactivation of the enzyme in methanol, ethanol and propanol solvents belonged to be noncompetitive type, while that in aldehyde and acetone solvents was mixed type. The Ki of methanol, ethanol and propanol were 5.36, 3.02,1.80 mol/dm^3 respectively. The K1 of aldehyde and acetone were determined to be 24.7 × 10^-3,1.66 mol/dm^3 respectively; and K1s were determined to be 56.2 × 10^-3,5.4 mol/dm^3 respectively.

关 键 词：海洋生物 碱性磷酸酶 实验研究 杂色鲍 失活作用 有机溶剂 

分 类 号：Q356.1[生物学—遗传学]