新型改性聚乳酸与成骨细胞相容性的研究  

Compatibility of a Novel Ethylenediamine Modified Polylactic Acid with Osteoblasts

作　　者：苏爱华[1] 王远亮[1] 罗彦凤[1] 吴科达[1] 

机构地区：[1]重庆大学生物工程学院生物力学与组织工程教育部重点实验室,重庆400044

出　　处：《生物医学工程学杂志》2005年第4期708-710,共3页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(30270395);教育部重点基金资助

摘　　要：探讨了成骨细胞与新型生物可降解材料乙二胺改性聚乳酸(EMPLA)的细胞相容性。在PLA、EMPLA及玻璃(对照组)上培养成骨细胞,采用细胞形态学观察法和细胞增殖法,在相差显微镜下观察细胞在上生长情况;分别于1、2、4、6d用MTT法记数,绘制生长曲线图。实验结果表明EMPLA组的成骨细胞比PLA组和对照组的形态好,增殖快,说明EMPLA比PLA表现出更好的细胞相容性,EMPLA在生物医学,特别是在组织工程领域存在着广泛的应用性。Biocompatibility of a newly developed ethylenediamine modified poly（DL -latic acid） （EMPLA） with osteoblasts was investigated by means of cell morphology and cell proliferation. Films of PLA and EMPLA were made by solvent casting. Osteoblasts obtained from crania of neonatal Wistar rats were cultured on surfaces of PLA and EMPLA, with glass as control. The cell morphology was observed by phase contrast microscope and the cell proliferation was determined by MTT assay. The morphology observations revealed that the osteoblasts cultured on EMPLA spread wider than those on PLA, and much more cells were confluent on EMPLA, compared to those on PLA and glass. The growth curves showed the osteoblasts on EMPLA grew faster than did those on PLA and glass. The results exhibited that the biocompatibility of EMPLA with osteoblasts is better than that of PLA and glass, which suggested wide applications of EMPLA in biomedical area, especially in tissue engineering.

关 键 词：乙二胺改性聚乳酸 成骨细胞 细胞相容性 生物可降解材料 细胞增殖法 细胞形态学 显微镜下观察 PLA MTT法 生物医学 

分 类 号：R318.08[医药卫生—生物医学工程]