一种用于骨组织工程研究的加载装置  

A New Loading Bioreactor for Bone Tissue-engineering Applications

作　　者：张春秋[1] 张西正[1] 王福荣[2] 吴金辉[1] 王月新 陆庆飞[1] 

机构地区：[1]军事医学科学院卫生装备研究所 [2]吉林大学白求恩医学院放射科,长春130031 [3]吉林省松江河林业集团公司,白山134504

出　　处：《生物医学工程学杂志》2005年第4期804-808,832,共6页Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(10172093);天津市科技发展基金项目(05YFJMJC01900);中国博士后基金资助项目(2003034177)

摘　　要：力学环境对骨内细胞的生物学行为有重要影响。无论是体内还是体外培养,骨组织的生长、发育都依赖力学环境。根据骨组织生理调节中的力学感知、传导、传质机理,研制一种用于骨组织工程研究的加载装置。该装置可为体外骨组织培养提供生理范围内不同大小、频率的应变及不同的应变波形,特别是对有一定强度的硬支架(与松质骨、密质骨强度相当)也能满足生理应变的要求。采用弹性较优的合成塑料作为参考支架进行有效性检测,结果表明在支架材料上此装置产生正常的生理应变,且重复精度高。装置使用智能材料-压电陶瓷作为动力来源,通过计算机控制实现了骨支架材料骨生理水平应变的精确控制。此装置将为工程化骨组织构建中载荷影响的研究提供方便。Bone cells live in an environment heavily influenced by mechanical force. The development of bone tissue is dependent on the environment that surrounds it, both in vivo and in vitro. A loading stimulator on research of bone tissue-engineering was developed based on the mechanism of mechanosensation, mechanotransduction and substance-mechanotransport in bone in vivo. The stimulator provides osteoblastsscaffolding composites with mechanical strains with more physiologic magnitude, frequency components, and waveform. It also achieves the mechanical environment particularly in hard scaffold enough strong llke cancellous bone. The device was tested using a reference scaffold made of better elastic plastic material. The experiment results showed that the device could be used in precision strain controls. Since the drive of the stimulator comes from the usage of smart material, piezoceramics, the strain at physiological level is controlled precisely. The stimulator provides a mechanical condition under which the effects of loading applied on bone tissue-engineering culture are conveniently investigated. Furthermore, after the stimulator is improved, it will be an appropriate bioreactor for bone tissue-engineering culture.

关 键 词：骨组织工程 应变 加载装置 支架 压电陶瓷 骨组织培养 组织工程研究 骨支架材料 计算机控制 生理调节 

分 类 号：R318.0[医药卫生—生物医学工程]