不同含水量菜豆种子老化过程中生理特性的研究  

Studies on Physiological Characteristics of Kidney Bean Seeds with Different Moisture Content in Aging Course

作　　者：李玉红[1] 陈鹏[2] 唐爱均[1] 王绮[1] 关志华[1] 程智慧[1] 

机构地区：[1]西北农林科技大学园艺学院,杨凌712100 [2]西北农林科技大学生命科学学院,杨凌712100

出　　处：《园艺学报》2005年第5期908-910,共3页Acta Horticulturae Sinica

基　　金：国家‘十五’科技攻关重大专项课题资助项目(2004BA516A09)

摘　　要：对不同含水量的菜豆种子(2·1%、3·4%、3·8%、6·9%和9·0%)老化过程中的生理特性进行了研究。结果表明,菜豆种子在含水量为3·8%时,发芽率,保护酶系统(SOD、POD和CAT)活性和脱氢酶活性明显高于其它含水量的种子,而脂氧合酶(LOX)活性和丙二醛(MDA)含量显著低于其它含水量的种子,表现出较高的生活力。此结果表明适度降低菜豆种子的含水量可以提高其抗老化劣变能力;不同生活力的菜豆种子POD同工酶酶谱表现不同,而SDS-PAGE蛋白质谱带则基本相同。Kidney bean seeds of different moisture contents （2. 1%, 3.4%, 3.8%, 6.9% and 9.0% ） were used to investigate the physiological characteristics in aging course. The results showed that the germination percent and the activity of SOD, POD, CAT and dehydrogenase were higher while LOX activity and MDA content were lower in 3.8% moisture content than other moisture content seeds. It showed more vigor than other seeds. Reducing the moisture content of kidney bean seeds could increase the ability of anti-aging. The isozymes of POD were different, while the protein bands were almost the same in kidney bean seeds of different vigor.

关 键 词：菜豆 种子老化 酶 丙二醛 POD同工酶 SDS—PAGE 

分 类 号：S643.1[农业科学—蔬菜学] S602.4[农业科学—园艺学]