凡纳滨对虾线粒体DNA COI基因片段序列  

Fragment Sequence of Mitochondrial DNA COI Gene of Litopenaeus vannamei

作　　者：郑连明[1] 林元烧[1] 曹文清[1] 方旅平[1] 周美玉[1] 王桂忠[1] 

机构地区：[1]厦门大学海洋与环境学院,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2005年第6期815-820,共6页Journal of Xiamen University：Natural Science

基　　金：国家自然科学基金(30471322)资助

摘　　要：以相应引物对凡纳滨对虾(Litopenaeus vannamei)线粒体DNA细胞色素氧化酶I亚基基因(mtDNA CO I)进行PCR扩增,经过基因重组、转化、克隆、筛选、DNA测序,得到709 bp的碱基片段.碱基组成A、C、G、T含量分别为198 bp(27.93%)、136 bp(19.18%)、129 bp(18.19%)2、46 bp(34.70%).与果蝇(Drosophila yakuba)的mtDNA CO I全序列比对,经分析发现:本实验获得的凡纳滨对虾mtCO I基因片段序列和GenBank上的同种序列(AY264901)都只是基因序列的一部分,二者之间有41 bp的重叠并可拼接,拼接后的总长为1 515 bp.证明本实验条件下获得的mtCO I基因片段确实来自凡纳滨对虾线粒体DNA,且本实验所用引物具有通用性.Litopenaeus vannamei which belong to the family Penaeidae （ Crustacea： Decapoda： Dendrobranchiata ） is the major species of shrimp farmed in aquaculture worldwide. With the development of the breeding scope, this cultured penaeus had suffered an epidemical disease which caused heavy mortality because of the deteriorating water environment, which causes the genetic depres- sion and the decrease of genetic biodiversity. So, the study on the protection of the germplasm resource and the community genetic structure of the L. vannamei has aroused the researcher＇s attention. In recent years, mitochondrial genome sequence and structure are widely used to provide information on phylogenetic relationships and on the genetic structure of populations and patterns of gene flow of the economical organism . Recently, analyses of penaeid mitochondrial genetics have been carried out using RFLP analysis and single-gene sequence analysis. In our research, mitochondrial cytochrome oxidase.subunit I （ CO I ） gene fragment of L. vannamei from Aoguanmariculture breeding field in Xiamen was successfully amplified via PCR. The PCR products were ligated into pGEM-T Easy Vector （Promega Co. ）, cloned and sequenced. 709 bp sequences of mtCO I were obtained. The contents of A, C, G andTwere 198bp（27.93%）,136 bp（19.18%）,129 bp（18.19%）,246 bp （34.70%）, respectively. A result was observed that the sequence we obtained and the same region of the sequence from GenBank （accession no. AY264901） were both a part of mtCO I suquence of L. vannamei, and they have a superposition of 41 bp of nucleotides and could link to be a 1515bp sequence, which prove the sequence we have got is really from mitochondrial genome of L. vannamei.

关 键 词：凡纳滨对虾 线粒体DNA CO I基因 序列 

分 类 号：P735[天文地球—海洋生物学] Q7[天文地球—海洋科学]