日本囊对虾线粒体DNA CO I基因片段序列分析  

Fragment Sequence Analysis of Mitochondrial DNA COI Gene of Marsupenaeus japonicus

作　　者：郑连明[1] 曹文清[1] 方旅平[1] 周美玉[1] 陈代斯[1] 林元烧[1] 王桂忠[1] 

机构地区：[1]厦门大学海洋与环境学院,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2005年第6期821-826,共6页Journal of Xiamen University：Natural Science

基　　金：国家自然科学基金(30471322)资助

摘　　要：以相应引物对日本囊对虾(Marsupenaeus japonicus)线粒体DNA细胞色素氧化酶I亚基基因(mtDNA CO I)进行PCR扩增,经过基因重组、转化、克隆、筛选、DNA测序,得到709 bp的碱基片断.碱基组成A、C、G、T含量分别为196bp(27.64%)、129 bp(18.19%)1、34 bp(18.90%)、250 bp(35.26%).与GenBank中斑节对虾(Penaeus monodon)的mtD-NA CO I全序列(AF217843)比对,经分析发现:本实验获得的日本囊对虾mtCO I基因片段序列和GenBank上的同种序列(AY264897)都只是该种CO I基因序列的一部分,二者之间有41 bp的重叠并可拼接,拼接后的总长为1 515 bp.Previous studies suggested that the mitochondrial cytochrome oxidase subunit I （CO I） gene was useful for analysis of taxonomic and phylogenetic relationships among Penaeidae species. Marsupenaeus japonicus, which mainly distributed in Southeast Sea of China is, of economical importance in both fishing and aquaculture in China. Considering the importance of this species, genetic information is vital to the design and implementation of sound fisheries management strategies and sustainable development of aquaculture. In this study,Mitochondrial cytochrome oxidase I gene fragment of Marsupenaeus japonicus was successfully amplified via PCR. The PCR products were ligated into pGEM-T Easy Vector （Promega Co. ） ,cloned and sequenced. 709 bp sequences of mtCO I were obtained. The contents of A,C,G and T were 196 bp（27. 64%） ,129 bp（18.19%） ,134 bp（18. 90%） ,250bp（35. 26%）,respectively. After comparison and analysis, a result was observed that the sequence we obtained and the same region of the sequence from GenBank （accession no. AY264897） were both a part of mtCO I suquence of M. japonicus,and they have a superposition of 41 bp of nucleotides and they could link to be a 1 515 bp sequence. This linked sequence verges on the complete mtCO I gene of Marsupenaeus japonicus.

关 键 词：日本囊对虾 线粒体DNA CO I基因 序列 

分 类 号：P735[天文地球—海洋生物学] Q7[天文地球—海洋科学]