重组鸡白细胞介素2质粒在体内的分布研究  

Biodistribution of recombinant plasmid encoding chicken IL-2 in vivo

作　　者：由振强[1] 于涟[1] 张朝政[1] 褚武英[1] 许健[1] 

机构地区：[1]浙江大学动物预防医学研究所浙江省动物预防医学重点实验室,杭州310029

出　　处：《中国预防兽医学报》2005年第6期522-526,共5页Chinese Journal of Preventive Veterinary Medicine

基　　金：浙江省科技重点攻关项目(011102465)

摘　　要：3周龄雏鸡肌肉接种200μg重组鸡白细胞介素2真核表达质粒(pCI-chIL-2).PCR法检测血清、心、肝、肺、脾、法氏囊和肌肉接种部位中pCI-chIL-2的残留情况,利用竞争PCR观察血清中重组质粒的动态分布,RT-PCR检测上述组织器官pCI-chIL-2的表达情况.结果表明:接种后2 h血清中即可检测到pCI-chIL-2,8 h达到高峰,7 d时已检测不到;pCI-chIL-2在上述组织器官中存在的时间比较长,1～14 d各组织器官中均有分布,19 d时肺和法氏囊中已检测不到;RT-PCR结果显示pCI-chIL-2转录的mRNA在各组织于不同时间均有存在.本研究为重组chIL-2作为免疫增强剂的应用提供体内试验数据.3-week-old chickens were administered with 200/_tg of recombinant chicken IL-2（pCI-chIL-2） by intramuscular injection. The recombinant plasmid in heart, liver, spleen, lung, bursal, inoculated muscle and serum was found by PCR and competitive PCR. To detect ILNA expression,at different time point total RNA was extracted from the tissues and reverse transcribed, then was amplified with primers. The Results showed that Serum distribution was observed at 2 h post inoculation, not detected at 7 d post inoculation and the highest distribution was found at 8 h. pCI-chIL-2 was also detectable in several tissues including heart, liver, lung, spleen, bursal and inoculated muscle. Except for bursal and lung, pCI-chIL-2 was still detectable in tissues at 19 d post administration. At the same time, a significant level of mRNA expression was observed in every tissue at different time.

关 键 词：鸡自细胞介素2(chIL-2) 分布 竞争PCR RT-PCR 

分 类 号：S852.4[农业科学—基础兽医学]