五种市售鸡传染性鼻炎灭活疫苗免疫效力比较  

Comparison of inactivated bivalent vaccines for avian infectious coryza

作　　者：孙惠玲[1] 苗得园[1] 陈晓峰[1] 张培君[1] 龚玉梅[1] 唐桂运 Anne-Lise Saint-Gerand 

机构地区：[1]北京市农林科学院畜牧兽医研究所,北京海淀100089 [2]梅里亚动物保健有限公司,200040

出　　处：《中国预防兽医学报》2005年第6期544-548,共5页Chinese Journal of Preventive Veterinary Medicine

摘　　要：本研究对国内市场上几个主要鸡传染性鼻炎灭活疫苗生产厂家生产的灭活疫苗免疫鸡只进行血清HI抗体水平测定和攻毒,比较不同公司所产疫苗效力的效力差异,并评估A、C型二价灭活疫苗两次免疫鸡群对国内B型分离株:DL-1株和最近从免疫失败鸡场分离到的SD-1株的交叉保护作用,分析免疫失败的原因。结果表明:A、D、E公司的疫苗免疫两次后,A型HI抗体阳性率分别为92.5%、100%和95%,滴度分别为33.9、55.1和59.9,攻毒保护率都是100%。C型HI抗体阳性率分别为72.5%3、8.5%和77.5%,滴度分别为11.4、2.7和27,攻毒保护率分别为80%、70%和80%。而B和C公司的疫苗免疫两次后A型HI抗体阳性率分别为59.4%7、7.1%,抗体滴度分别为5.4和21.8,攻毒保护率分别为50%和66.7%;C型HI抗体阳性率分别为54.1%和51.4%,抗体滴度分别为6.1和6.8,攻毒保护率分别为38.3%和50%。在五个疫苗产品中,以A、D、E的保护效力较好,B、C产品效力较差。另外,A、C型二价灭活疫苗免疫后不能对B型菌的攻击提供保护,其A、C型HI抗体阳性率、抗体滴度与对B型菌攻毒保护率无相关性。Inactivated bivalent vaccines for avian infectious coryza from five different producers were studied and their immune efficacy were also tested and compared by HI antibody titer and protection against challenge. Another objective is to estimate the cross-protection of the chickens with 2 dose A ＋ C vaccination against serovar a B serovar isolates： DL-1 and a A serovar strain isolated in Shandong. The results showed that, with 2 dose vaccination,the positive rates of HI antibodies to serovar A of A, D, E producers＇ vaccines were 92.5 %, 100 % and 95 % respectively, the HI titers were 33.9,55.1 and 59.9 respectively, and protection against challenge with serovar A were all 100 % .The positive rates of HI antibodies to serovar C were 72.5 % ,38.5 % and 77.5 % respectively,the HI titers were 11.4,2.7 and 27 respectively, the protection against challenge with serovar C were 80 % ,70 % and 80 % respectively. At the same time,The positive rates of HI antibodies to serovar A of B,C producers＇ vaccines were 59.4 % and 77.1% respectively, the HI titers were 5.4 and 21.8 respectively, the protection against challenge with serovar A were 50 % and 66.7 % respectively. The positive rates of HI antibodies to serovar C were 54.15 % and 51.4 % respectively,the HI titers were 6.1 and 6.8 respectively,and protection against challenge with serovar C were 38.3 % and 50 % respectively. There was no correlation between the A/C serovar specific HI titer antibody and the protection against challenge with serovar B.

关 键 词：副鸡嗜血杆菌 鸡传染性鼻炎灭活疫苗 HI抗体 

分 类 号：S852.62[农业科学—基础兽医学]