接骨木中的三萜类化合物及其对类成骨细胞UMR106的作用  

Studies of triterpenoids isolated from Sambucus williamsii Hance and their effects on UMR106 cell proliferation and alkaline phosphatase activity

作　　者：杨序娟[1] 王乃利[1] 黄文秀[2] 陈新滋[2] 姚新生[1] 

机构地区：[1]沈阳药科大学中药学院,辽宁沈阳110016 [2]香港理工大学应用生物及化学科技学系

出　　处：《沈阳药科大学学报》2005年第6期449-452,457,共5页Journal of Shenyang Pharmaceutical University

基　　金：香港特别行政区大学教育资助委员会TheareaofExcellenceScheme资助项目(AOE/r10/01)

摘　　要：目的用大鼠类成骨细胞UMR106的增殖作为活性追踪指标,对接骨木茎枝的体积分数为60%的乙醇提取物进行抗骨质疏松活性成分的追踪分离;初步考察化合物对UMR106细胞增殖、分化的影响.方法运用各种现代分离手段对活性部位进行追踪分离,用光谱学方法对得到的化合物进行结构鉴定;用比色法测定UMR106细胞的增殖和碱性磷酸酶活性.结果分离得到了5个三萜苷元类化合物,包括白桦醇(betulin,1)、白桦酸(betulinic acid,2)、齐墩果酸(oleanolic acid,3)、熊果酸(ursolic acid,4)、α-香树脂醇(α -amyrin,5),同时还分离到了2个甾醇类化合物,豆甾醇(stigmasterol,6)、胡罗卜苷(sitosterol-3-glucoside,7).结论化合物1～3均为首次从接骨木中分离得到,其中化合物1、2可以促进大鼠类成骨细胞UMR106的增殖,化合物1对UMR106细胞碱性磷酸酶的活性也有促进作用.Objective To study the bioactive constituents from the 60 % ethanol extract of the stems of Sambucus wiilliamsii Hance while stimulating UMR106 cell proliferation was used as in vitro marker to guide the isolation procedure;to determine the effects of isolated compounds on UMR106 cell proliferation and alkaline phosphatase activities. Methods Isolation procedure was carried out by column chromatography, medium pressure column chromatography and HPLC; structures of isolated compounds were identified by physiochemical properties and spectroscopic methods; effects of isolated compounds on UMR106 cell proliferation and ALP activities were measured by colorimetry. Results Isolation of the 60 % ethanol extract of the stems of Sambucus williamsii Hance affored five triterpenes and 2 steroids, including betulin（1）, betulinic acid （2）, oleanolic acid （ 3 ）, ursolic acid （4）, α -amyrin （ 5 ）, stigmasterol （ 6 ） and sitosterol-3-glucoside （7）. Conclusions Betulin（1）, betulinic acid（2） and oleanolic acid（3） are isolated from Sambucus williamsii Hance for the first time. Betulin （1） and betulinic acid （2） could stimulate UMR106 cell proliferation, and betulin（1） could also induce ALP activities in UMR106 cell.

关 键 词：UMR106细胞 接骨木 抗骨质疏松 

分 类 号：R96[医药卫生—药理学] R284.1[医药卫生—药学]