微小病变型肾病患儿外周血淋巴细胞糖皮质激素膜受体与激素对其凋亡、增殖的作用  

Relationship of Membrane Glucocorticoid Receptors in Peripheral Blood Lymphocytes from Minimal Change Nephrotic Children and Influence of Glucocorticoid on Apoptosis and Proliferation

作　　者：刘晓鸣[1] 卢思广[2] 王峥[1] 薛凌宇[2] 

机构地区：[1]四川大学华西第二医院儿科,成都610041 [2]徐州医学院附属医院儿科,江苏徐州221002

出　　处：《实用儿科临床杂志》2005年第11期1096-1098,共3页Journal of Applied Clinical Pediatrics

摘　　要：目的探讨微小病变型肾病(MCNS)外周血淋巴细胞(PBLs)糖皮质激素膜受体(mGR)表达及其与糖皮质激素(GC)对PBLs调亡、增殖作用的关系,并观察GC对mGR表达影响。方法分别以间接免疫荧光、碘化丙啶染色及3H-胸腺嘧啶核苷(3H-TdR)掺入法分析MCNS和NMCNS患儿mGR表达、PBLs凋亡及增殖情况。结果1.MCNS患儿mGR表达高于非微小病变组(NMCNS)及正常组,临床GC治疗后降低;2.mGR表达与10-7mol/L地塞米松(DEX)诱导PBLs凋亡率及抑制其3H-TdR掺入率呈正相关。结论MCNS患儿mGR表达增高。mGR可能参与GC诱导PBLs凋亡和(或)抑制其增殖作用。GC对mGR表达有下调作用。Objective To study the expression of membrane glucocorticoid receptors（mGR）, the correlation between mGR and glucocorticoid（GC）＇s effects on apoptosis and proliferation of peripheral blood lymphocytes（PBLs） in minimal change nephrotic syndrome（MCNS）and the influence of GC on mGR. Methods MCNS, nonminimal change NS（NMCNS） and healthy children were involved in this study. Indirect immune fluorescence and flow cytometry were used to examine the percentages of positive mGR lymphocytes; the apoptosis of PBLs was measured by propidium iodide（PI） staining and the proliferation of PBLs was investigated by ^3H-TdR incorporation. Results 1. mGR expression in MCNS was higher than that in NMCNS and healthy control, but it was reduced af- ter clinical GC therapy（P〈0.05）. 2. The mGR expression in MCNS correlated positively with the apoptotic rate induced by 10^-7 mol/ L Dexamethason（DEX） and the percentage of ^3H - TdR incorporation inhibited by 10^-7 mol/L DEX. Conclusions Expression of mGR in MCNS was increased, mGR may be an important contributor to GC＇s inducing apoptosis and inhibiting proliferation. GC can down-regulate mGR expression.

关 键 词：肾病 脂性 淋巴细胞 糖皮质激素类 细胞凋亡 

分 类 号：R726.91[医药卫生—儿科]