角膜脱细胞基质的特性研究  

Study on the characteristics of a cellular cornea martrix

作　　者：刘晓霞[1] 陈建苏[1] 丁勇[2] 黎明庆[3] 

机构地区：[1]暨南大学医学院眼科研究室,广州510632 [2]暨南大学附属第一医院,广州510632 [3]暨南大学生物力学材料实验室,广州510632

出　　处：《广东医学》2005年第11期1463-1464,共2页Guangdong Medical Journal

基　　金：国家自然科学基金资助项目(编号:30371519);广东省自然科学基金资助项目(编号:04010432)

摘　　要：目的采用适当的角膜脱细胞方法获得角膜脱细胞基质,并对其进行特性研究。方法采用胰酶-冰冻法对新鲜兔角膜(全层和分层)进行脱细胞处理,再进行组织学检测及生物学特性力学特性、含水率、膨胀率分析。结果用胰酶-冰冻法获得的兔角膜全层和分层脱细胞基质未见细胞成分,细胞外基质的结构未见明显破坏,脱细胞基质在生物力力学特性、含水率、膨胀率上与正常角膜接近,透光率低于新鲜角膜。结论用胰酶-冰冻法获得的脱细胞基质与自然角膜接近,可作为组织工程角膜支架材料和角膜替代物。Objective To obtain acellular corneal martrix（ACCM） via extraction of cell from cornea and to study its biological characteristics. Methods Fresh rabbit cornea were shaken in 0.25% trypsin for 1 hour and were frozen for 3 days, Cornea were stained with HE and their characteristics were examined, Results There were no celluar components in ACCM and there were no distinct differences in mechanical characteristics, water-content rate and expansive rate between ACCM and cornea. The light trasmittance of ACCM was lower compared to cornea. Conclusion ACCM can be used as a biological scaffold for tissue engineering and corneal substitute.

关 键 词：脱细胞基质 特性研究 兔角膜 力学特性 新鲜角膜 脱细胞处理 生物学特性 组织学检测 细胞外基质 冰冻法 细胞成分 正常角膜 支架材料 组织工程 含水率 膨胀率 胰酶 生物力 透光率 替代物 全层 

分 类 号：R318.08[医药卫生—生物医学工程] R779.65[医药卫生—基础医学]