发酵条件对木霉菌株T23的菌丝生长及几丁质酶活性的影响  

Influence of Fermentation Conditions on Hyphae Growth and Chitinase Activity of Trichoderma Strain of T23

作　　者：吕淑霞[1] 于晓丹[1] 张彩霞[1] 陈捷[2] 

机构地区：[1]沈阳农业大学生物科学技术学院,沈阳110161 [2]上海交通大学生物与农业学院,上海201101

出　　处：《沈阳农业大学学报》2005年第3期332-335,共4页Journal of Shenyang Agricultural University

基　　金：辽宁省博士启动基金项目(20021042)

摘　　要：为使木霉生防制剂实现工业化生产,本试验对木霉菌株T23的最适菌丝生长和最佳产几丁质酶的发酵条件进行了研究。试验结果得出T23的最佳发酵条件以PDB为培养基,在250mL三角瓶中50mL装瓶量,接种量为6%,起始pH值为5,温度25℃离心机转速180r.min-1,培养4d,发酵液中菌丝产量和几丁质酶活分别达到10.64g.L-1和421.3U.mL-1。The optimal hyphae growth conditions of strain of Trichodema Harianum T23 and its optimal fermentation conditions for chitinase-producing was performed in order to provide the scientific bases for industrial production of this biocontrol preparation. The results showed that the optimal conditions were as follows： 50mL medium of potato dextrose broth filled in 250mL flask with 6% inoculum of the culture, initial pH5, at 25℃, 180rpm incubation for 4d. The hyphae quantity and chitinase activity were 10.64g·L^-1 and 421.3U·mL^-1 respectively.

关 键 词：木霉 几丁质酶 发酵 生物防治制剂 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]