神经干细胞移植对大鼠脊髓半切空洞损伤的修复作用  

Repairing effects of neural stem cells transplantation on the semi-cut cavity of spinal cord in rats

作　　者：刘媛 [1] 李庭梅  龙在云 [1] 曾琳 [1] 陈恒胜 [1] 伍亚民  李应玉 [1] 杨恒文 [1] 廖维宏 [1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所三室,创伤、烧伤与复合伤国家重点实验室,重庆市大坪长江支路10号,400042 [2]成都市铁路局中心医院神经内科

出　　处：《中国矫形外科杂志》2005年第20期1573-1576,共4页Orthopedic Journal of China

基　　金：国家自然科学基金(编号:30171068);重庆市院士基金资助(合同号:7671)

摘　　要：[目的]观察神经干细胞移植后对大鼠损伤脊髓形态和功能的修复作用。[方法]30只Wistar大鼠,分为半切洞损伤组、DHanks液对照组和神经干细胞移植治疗组。4、8周后取损伤部位脊髓组织进行HE、Nissl、Holmes银染观察神经纤维和神经细胞的再生情况。同时在伤后1、4、8周进行伤侧下肢MEP和SEP检测,了解感觉和运动功能的恢复情况。[结果](1)治疗组4周后,分化的细胞基本闭合空洞,形成一空腔。8周时,在损伤处可见大量星形胶质细胞,及少数长出突起、存活的神经元,而且移植物与宿主之间在形态上形成纤维联系;(2)术后1周各组运动诱发电位和感觉诱发电位峰潜时明显延长,无显著差异性,手术后4、8周,C组的峰潜时较A、B组明显缩短,有显著的差异性(P<0.01)。[结论]神经干细胞移植到损伤脊髓组织后,能够存活、分化并从结构和功能上较好地修复组织缺损区域。[Objective] To observe the morphological, and functional repairing effects of NSCT（neural stem cells transplantation） on SCI（spinal cord injury） in rats. [Method] Thirty Wistar rats were divided into three groups randomly： semi-cut cavity injured group（group A）, D- Hanks controlled group（ group B） and NSCT group（ group C）. All the SCI tissues in different groups were stained with HE, Nissl to observe the regeneration effects of neural fibres and cells at 4 and 8 weeks after injury. MEP （motor evoked potential） and SEP （ somatosensory evoked potential ） of lower limb were examined to learn the rehabilitation of sensory and motor function at 1,4 and 8 weeks after injury. [ Result] 1. The cavity was basically closed by differentiated cells. At 8 week after injury, there were much astrocytes and some surviving neurons with dendrites, which formed fibrous contact between grafts and the hosts in morphology ; 2. The peak of latencies became much longer than before, which showed no differences between all groups at 1 week after injury. The peak of latency in group C was much shorter than those in group A and B at 4 and 8 weeks with significant differences （P 〈 0.01 ）. [Conclusion]The neural stem cells transplanted into the injured spinal cord can survive, differentiate and repair the injured area in terms of structure and function.

关 键 词：神经干细胞 移植 脊髓损伤 结构修复 WISTAR大鼠 神经干细胞移植 损伤脊髓 修复作用 脊髓半切 空洞 

分 类 号：R651.2[医药卫生—外科学]