聚乙烯醇/羟基磷灰石复合水凝胶移植修复兔膝关节软骨缺损(英文)  

Experimental study of rabbit knee joint articular cartilage defects repaired with transplantation of polyvinyl alcohol / hydroxyapatite composite hydrogel

作　　者：卢华定[1] 蔡道章[1] 刘青[2] 王迎军[2] 郑裕东[2] 

机构地区：[1]中山大学附属第三医院骨科,广东省广州市510630 [2]华南理工大学材料学院,广东省广州市510640

出　　处：《中国临床康复》2005年第34期184-186,189,共4页Chinese Journal of Clinical Rehabilitation

基　　金：广东省医学科研项目(B2002040)资助~~

摘　　要：背景:目前临床治疗由创伤、骨关节炎等引起的关节软骨病损的方法不能使受损的软骨在形态学、生化和生物力学方面恢复至正常,修复组织不能达到良好的远期疗效,常在短期内发生退行性变。目的:探讨聚乙烯醇/羟基磷灰石复合水凝胶移植修复兔膝关节软骨缺损的疗效及了解其组织相容性。设计:随机对照动物实验。单位:中山大学附属第三医院动物实验中心。材料:实验于2003-10/2004-04在中山大学附属第三医院动物实验中心完成。成年健康新西兰白兔20只,雄性12只,雌性8只,体质量2.1～3.0kg,由中山大学动物实验中心提供.饲养环境温度20℃,相对湿度40%。将兔子随机分成空白对照组(n=6)和实验组(n=14)。方法:采用溶胶凝胶法原位复合制备聚乙烯醇/羟基磷灰石水凝胶,通过体外力学性能测试,采用日本岛津公司生产的AG-1型万能电子拉力试验机对材料的拉伸性能进行测试,拉伸速度为500mm/min。空白对照组不作任何处理,实验组于软骨缺损处植入圆柱形聚乙烯醇/羟基磷灰石复合水凝胶,嵌压固定。术后动物单笼关养,所有术肢不予固定,任其自由活动,常规用青、链霉素抗感染治疗。术后第4,8,12周空气栓塞法每批处死6～7只兔子,取患膝关节作大体、光镜观察。主要观察指标:主要结局:两组家兔膝关节软骨缺损的大体和组织学改变。次要结局:聚乙烯醇/羟基磷灰石复合水凝胶的体外拉伸强度与浸泡时间的关系。结果:①两组家兔膝关节软骨缺损的大体和组织学改变:术后4周,实验组的缺损由聚乙烯醇/羟基磷灰石充填,材料与软骨下骨连接无间隙,术后12周,植入材料与软骨交界面有大量的软骨细胞增殖,未见软骨退变,植入材料与软骨下骨连接紧密,有骨样组织长入;空白对照组缺损主要由纤维肉芽组织修复。②聚乙烯醇/羟基磷灰石复合水凝胶的体外拉伸强度与浸泡时间BACKGROUND： At present, the therapy to treat articular cartilage lesions caused by trauma, osteoarthrhis and other diseases fails to repair the damaged cartilage to its normal state morphologically, biochemically and biomechanically and for the repaired tissues, good long-term effect doesn＇t happen and retrograde degeneration happens in a short period. OBJECTIVE： To study the curative effect of transplantation of polyvinyl alcohol/hydroxyapatite （PVA/HA） composite hydrogel to repair rabbit knee joint articular cartilage defects and to learn about its biocompatibility with host tissues. DESIGN： A randomized controlled experimental anim.,d study. SETTING： The Animal experimental Center of the Third Affiliated Hospital of Sun Yat-sen University. MATERIALS： The experiment was conducted at the Animal Experimental Center of the Third Affiliated Hospital of Sun Yat-san University. Totally 20 adult healthy New Zealand white rabbits including 12 males and 8 females, with a body mass of 2.1 to 3.0 kg were provided by the Animal Experimental Center of Sun Yat-sen University. All animals were bred in an environment of 20℃ and 40% of humidity. The rats were randomized into the blank control group （n=6） and the experimental group （n=14）. METHODS： Polyvinyl alcohol/hydroxyapatite composite hydrogel was made by sol-gel method of hydroxyapatite powder. The tensility was tested at a pulling speed of 500 mm/min by AG-1 omnipotence electronic pulling tester （produced by Shimadz Co. of Japan） through mechanical property test in vitro. Rabbits in the control group received no other procedures while the rabbits in the experimental group had their knee joint articular cartilage defects repaired with transplantation of cylindrical PVA/HA composite hydrogel, fixed by inlaying the hydrogel into the defect. Fed in the cage alone postoperatively, all aniraals were permitted to move without any immobilization to the operated limbs. Routine therapy of benzylpenicillin and streptomycin anti-infection was performe

关 键 词：软骨 关节 生物相容性材料 羟基磷灰石类 移植 

分 类 号：R684[医药卫生—骨科学]