昆明山海棠总生物碱对肺腺癌A549细胞株增殖抑制作用的实验研究  

Inhibition on proliferation of human lung adenocarcinoma cell line A549 by alkaloids from tripterygium hypoglaucum hutch

作　　者：刘乐斌[1] 杨录军[1] 刘胜学[1] 刘晋祎[1] 敖琳[1] 黄晓春[1] 曹佳[1] 

机构地区：[1]第三军医大学军事预防医学院卫生毒理学教研室,重庆400038

出　　处：《第三军医大学学报》2005年第19期1915-1917,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金资助项目(C30100241)~~

摘　　要：目的观察昆明山海棠总生物碱(alkaloidsfromtripterygiumhypoglaucumhutch,THHa)对人肺腺癌细胞系A549体外生长的影响。方法采用MTT比色法检测THHa对A549细胞生长的抑制作用,测定其吸光度D(490)值;用倒置和荧光显微镜观察药物作用后A549细胞形态的变化;流式细胞仪检测细胞凋亡率。结果随着THHa浓度的增加和作用时间的延长,A549细胞增殖受到抑制,抑制率逐渐升高,具有浓度和时间依赖性。流式细胞仪结果显示,随着处理浓度和作用时间的增加,细胞的凋亡率逐渐升高。结论THHa能明显抑制A549细胞增殖,并可诱导A549细胞凋亡。Objective To observe the effect of the alkaloids from tripterygium hypoglaucum hutch （THHa） on cell proliferation and cell apoptosis in the human lung adenocarcinoma cell line A549. Methods A549 cells were eoeultured with THHa solution of different concentrations （0, 12.5, 25, 50, 100, 200 μg/ ml） for 24, 48, 72 h and the inhibition rate of A549 cells was measured by MTT assay. Invert microscope and fluorescence microscope were used to observe the morphologic changes of A549 cells stained by Hochest 33342. The amount of apoptotic cells was measured by flow cytometry. Results The proliferation of A549 cells cocultured with THHa was obviously inhibited as compared with the control group. The inhibition rate of A549 cells treated with THHa increased in a time-and concentration-dependent manner and there was a significant difference between the ceils cocultured with THHa and the control cells （P 〈 0.05）. Morphological changes of typical apoptosis in A549 cells by Hochest 33342 staining were observed under fluorescent microscope. The cell apoptosis rate by flow cytometry increased in a concentration-dependent manner. Conclusion THHa inhibits the proliferation of A549 cells and induces the apoptosis of the human lung adenocarcinoma cell line A549 in vitro.

关 键 词：昆明山海棠 肺腺癌A549细胞 增殖 凋亡 

分 类 号：R285.5[医药卫生—中药学] R329.28[医药卫生—中医学]