重型肝炎病人血浆对体外培养HepG2细胞生长和解毒功能的影响  

Effect of plasma from patients with severe hepatitis on growth and detoxification of HepG2 cells

作　　者：程永波[1] 陈志[1] 王英杰[1] 张世昌[1] 刘俊[1] 

机构地区：[1]第三军医大学西南医院全军感染病研究所,重庆400038

出　　处：《第三军医大学学报》2005年第19期1953-1956,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金资助项目(30027001)~~

摘　　要：目的了解重型肝炎病人血浆(severe-hepatitisplasma,SHP)对HepG2细胞生长和解毒功能的影响,为保持生物人工肝反应器内肝细胞活性研究提供实验依据。方法复苏培养HepG2细胞,通过细胞总蛋白含量和细胞周期测定、形态变化观察了解SHP对细胞生长的影响,从细胞周期角度探讨这种影响的机制。同时,通过谷胱甘肽含量检测了解SHP对细胞解毒功能的影响。结果①SHP培养HepG2细胞总蛋白合成显著下降(F=46.852,P<0.01),浓度越高下降幅度越大,形态观察细胞细小,明显稀疏;终止SHP培养,换用普通培养液后细胞仍有LDH漏出,24h时高出对照组近1倍;细胞生长周期测定发现SHP培养的HepG2细胞S期所占比例比对照组增高,第1天差异非常显著(F=92.239,P<0.01),第3天只有100%SHP组增幅差异非常显著(多重比较P<0.01)。②各种浓度SHP培养的HepG2细胞谷胱甘肽含量第1天均显著升高(F=10.097,P<0.01),但第3天开始下降;不同浓度间GSH含量差异显著(F=8.097,P<0.05)。结论SHP抑制HepG2细胞生长,短时间内可促进HepG2细胞解毒,但随时间延长解毒功能耗竭。Objective To investigate the effect of plasma from severe hepatitis patients （SHP） on the growth and detoxification of HepG2 cells. Methods HepG2 cells were recovered and cultured in SHP of different concentration. Total protein, the leakage of LDH, the morphologic changes, cell cycles, glutathione of HepG2 cells were investigated. Results The synthesis of total protein was inhibited （ F = 46. 852, P 〈0.01 ） in 100% SHP group. Exposed to SHP for 24 h, LDH leakage of HepG2 cells was approximately one-fold higher than that of controls and the cell morphology turned to be thin and sparse. The percentage of cells at S phase was significantly increased on day 1 （ F =92. 239, P 〈0.01 ）. Exposure of HepG2 cells to SHP resulted in an increase （ F = 10. 097, P 〈0.01 ） in the GSH content within 24 h as compared to that of controls. On day 3, the GSH level declined to below the levels of controls. The statistical difference of GSH in different-concentration SHP groups was significant （F = 8. 097, P 〈 0.05）. Conclusion SHP inhibits the growth of HepG2 cells, increases the detoxification in 24 h but declines after 3 days.

关 键 词：HEPG2细胞 重型肝炎 血浆 细胞周期 解毒 

分 类 号：R322.47[医药卫生—人体解剖和组织胚胎学] R329.25[医药卫生—基础医学]