纤维蛋白溶解酶诱导兔眼玻璃体后脱离  

Plasmin-induced posterior vitreous detachment

作　　者：黄玲[1] 王丽丽[1] 王海燕[1] 郝安平[1] 张妍春[1] 

机构地区：[1]西安市第四医院眼科,710004

出　　处：《中华眼底病杂志》2005年第6期388-390,共3页Chinese Journal of Ocular Fundus Diseases

基　　金：陕西省自然科学研究基金资助项目(200C280)

摘　　要：目的研究纤维蛋白溶解酶分别联合透明质酸酶和气体六氟化硫(SF6)诱导兔眼玻璃体后脱离(PVD)的效果。方法18只健康成年青紫兰兔随机分为A、B、C3组,每组6只兔。右眼均为实验眼,左眼为对照眼。A、B、C组实验眼玻璃体腔内分别注射纤溶酶1U(浓度10U/ml)、纤溶酶1U和透明质酸酶20U(浓度200U/ml)、纤溶酶1U和SF60.5ml;对照眼玻璃体腔内注射眼用平衡盐溶液(BSS)0.1ml。注药前后行间接检眼镜、裂隙灯和VOLK+90D前置镜及B型超声和视网膜电图(ERG)检查。最后取所有兔眼行光学显微镜、扫描电子显微镜和透射电子显微镜观察。结果扫描电子显微镜观察结果A组实验眼中有2只眼后极部发生不完全性PVD,发生率为33.3%;B、C两组实验眼中分别各有4只眼发生完全性PVD,发生率为66.7%;B、C两组实验眼出现PVD的阳性发生率与A组比较,差异有统计学意义(P<0.05)。3组实验眼注药后ERG振幅与手术前及对照眼比较差异均无统计学意义(P>0.05)。光学显微镜和透射电子显微镜观察结果显示,视网膜组织结构无明显异常改变。结论纤溶酶联合透明质酸酶或SF6较单独采用纤溶酶更能迅速有效的诱发完全性PVD且对视网膜无明显的毒性作用。Objective To detect the effects oi plasmin combined with hyaluronidase or hexafluoride SF6 on inducing posterior vitreous detachment （PVD）. Methods Eighteen young pigmented rabbits were randomly divided into group A, B, and C with 6 rabbits in each. All of the right eyes were the experimental eyes and the left ones was the control. The right eyes in group A, B, and C were injected with plasmin 1 U, plasmin 1 U and hyaluronidase 20 U, and plasmin 1 U and SF6 0.5 ml, respectively; while all of the left eyes underwent intra vitreous injection with balanced salt solution 0. 1 ml. The eyes were observed by indirect ophthalmoscopy, slit lamp examination, biomicroscopy, B uhrasonography, and electroretinography （ERG） before and after injection respectively. At last, the retinal sections were examined by light microscopy, scanning and transmission electron microscopy. Results The results of scanning microscopy showed incomplete PVD in 2 （33.3%） experimental eyes in group A, and complete PVD in 4 （66.7%） experimental eyes in both group B and C, and the positive rate of PVD in both group B and C significantly differed from that in group A （P〈0.05）. The b-wave amplitudes of ERG in the three groups after injection didn＇t differ much from that in the control group or before the injection（P〉0.05）. The results of transmission electron microscopy and light microscopy indicated unchanged retinal structure. Conclusions Compared with the application of only plasmin, plasmin combined with hyaluronidase or hexafluoride SF6 can induce complete PVD more efficiently and do no harm to the retina.

关 键 词：纤溶酶 透明质酸葡萄胺酶 玻璃体脱离 疾病模型 动物 

分 类 号：R776.4[医药卫生—眼科]