Y-染色体特异性探针追踪骨髓间质干细胞移植后转归的实验研究  

An experimental study on the conversion of mesenchymal stem cells after transplantation followed up by Y chromosome specific probe

作　　者：徐栋梁[1] 杨远良[1] 谭本前[1] 李佛保[1] 

机构地区：[1]中山大学附属第一医院骨科,广州市510080

出　　处：《中华显微外科杂志》2005年第4期331-333,i0003,共4页Chinese Journal of Microsurgery

基　　金：广东省科技厅重点项目基金(9622050-03)

摘　　要：目的追踪观察骨髓间质干细胞(MSC)移植后细胞的存活、转归和功能保存状况。方法将雄性SD大鼠分离培养的MSC移植到雌性SD大鼠骨缺损部位,动态取不同时段的组织,采用地高辛标记的Y染色体特异性DNA探针进行原位杂交,观察移植细胞的分布、数量和功能状况。结果同种异体MSC移植后,可在受体骨缺损局部生存、增殖,形成骨痂数量多、质量好。干细胞移植90 d后仍存活,可分布于骨髓和新骨组织。结论同种异体MSC移植后可长期存活于骨髓和新骨组织并保存成骨特性。Objective To observe the survival,conversion and function preservation of mesenchymal stem cells （MSC） after transplantation. Methods MSCs from male Sprague-Dawley （SD） rats were isolated and cultured,then were transplanted to bone defect of female SD rats. Tissues were obtained at defferent periolds. Hybridization in situ was performed on the tissues using Y chromosome specific DNA probe labeled with digoxin to observe the transplanted cells on distribution,quantity and function. Results Allogeneic MSCs could survive and proliferate in the bone defect of recipients and formed bony callus with large quantity and good quality. After 90 days of graft, stem cells still survived and distributed in marrow and newly formed bone tissue. Conclusion After transplantation,allogeneic MSCs have a long-term surviving in marrow and new bone tissue and maintain the characteristic of ossification.

关 键 词：骨髓间质干细胞 移植 Y-染色体 原位杂交 追踪观察 移植后 特异性探针 转归 实验研 特异性DNA探针 

分 类 号：R457.7[医药卫生—治疗学]