外源黄体酮对孕鼠子宫形态学改变及子宫内膜凝集素受体表达的研究  

Effect of progesteroneon on the morphology in uterusand ovary and the expression of the receptorsof lectins

作　　者：赵伟[1] 安利国[1] 马保华[2] 

机构地区：[1]山东师范大学生命科学院,山东济南250012 [2]山东大学医学院

出　　处：《山东医药》2005年第31期13-15,共3页Shandong Medical Journal

基　　金：山东省自然科学基金计划(Y2004C08)

摘　　要：目的研究外源黄体酮对卵巢、子宫形态学的影响机理和对子宫黏膜上皮细胞凝集素受体表达规律的影响.方法对妊娠金黄地鼠腹腔注射黄体酮作为动物模型,采用一般微观形态结构和电镜超微形态结构观察、亲和免疫细胞化学技术凝集素分子探针观察、同位素放射免疫对动物血清激素进行定量分析、停药后实验动物动情周期恢复实验观察.结果①动物注射黄体酮停药第6天后卵巢、子宫微观和超微观形态学发生明显改变;②停药第6天后凝集素分子探针测定子宫黏膜上皮细胞,ConA、PNA、UEA等凝集素受体含量出现明显变化,随后变化逐渐恢复;③停药第5～6天后同位素放射免疫法测定血清激素浓度与对照组比较有显著性差异(P＜0.05);④停药后第10天实验动物可恢复动情周期.结论外源黄体酮可通过改变妊娠动物卵巢、子宫内膜的形态结构和内膜上皮细胞合成凝集素受体含量,明显抑制胚胎生存作用;外源黄体酮抑制动情周期的生物学效应是可逆的.Objective： To study the effect of proyester oneon on the morphology in uterus and ovary and the expression of the receptors of lectins. Methods： The mated female hamster were given the progesteroneon in dose of 2. 5mg/（kg · d） on 3th day of gestation and were observed the change of lectin receptor in the endometrial epithelial cell of the uterus. Light microscopy and electron microscopy,affinity cytochemistry and the radioimmunoassay （RIA） were adopted. Results： The irregularity of epithelium, the disappearance of uterine gland and the bleeding in the endometrium of uterus could be seen. The disappearance of epithelial cilia, the increase of mitochondria and ribosome in endometrium could be seen by electron microscope. The staining intensity for the lectin receptors of ConA, PNA and UEN on the endometrium of experimental, group were significantly higher than those of the controls. The stainning intensity for ConA in experimental group was significantly higher than that of the controls and the stainning reaction was weaken for PNA and UEA. The FSH, LH, E2 and progestogen concns in serum of experimental group showed difference with that of the control group by RIA. Conclusion： Progesteroneon may change ectin receptor on the surface of the endometrial epithelial cell. After 8th days fertility of the treated hamsters were recovered.

关 键 词：外源黄体酮 形态学 凝集素 受体 胚胎 

分 类 号：R911.2[医药卫生—药学]