RP-HPLC法测定石韦药材中绿原酸的含量  

Determination of Chlorogenic Acid in Crude Folium Pyrrosiae by RP-HPLC

作　　者：孙全明[1] 朱朝德[1] 李春雨[1] 张康生[1] 胥道宝 张转平 王静[1] 

机构地区：[1]安康市药品检验所,陕西安康725000

出　　处：《中国药品标准》2005年第5期21-23,共3页Drug Standards of China

基　　金：<中国药典>2005年版科研项目

摘　　要：目的:建立石韦药材中绿原酸的含量测定方法。方法:采用RP-HPLC法,使用C18柱,以乙腈-0.5%磷酸溶液(11∶89)为流动相;检测波长为326nm;流速为1.0ml/min。结果:绿原酸在0.126～0.632μg范围内线性关系良好(r=0.9998),平均回收率为99.37%(RSD=1.27%)。结论:本法简便,快速,灵敏,准确,重现性好,可用于石韦药材的质量控制。Objective：To establish a method for the determination of Chlorogenic Acid in crude Folium pyrrosiae. Method： Kromasil C18（250mm×4.6mm,5μm）column was used. The mobile phase consisted of MeCN-0.5% H3PO4（11 ： 89）. The detection wavelength was at 326nm. The flow rate was 1.0ml/min. Result： The calibration curve of Chlorogenic Acid was in good linearity over the range of 0. 126-0. 632μg（r = 0. 9998）. The mean recovery was 99.37% （RSD=1. 27%）. Conclusion： The method is simple ,quick,sensitive ,accurate ,reproducible and can be used to control the quality of crude Folium pyrrosiae.

关 键 词：RP-HPLC 石韦药材 绿原酸 RP-HPLC法测定 含量测定方法 中绿原酸 石韦药材 C18柱 检测波长 线性关系 质量控制 流动相 

分 类 号：R284.1[医药卫生—中药学] R927.2[医药卫生—中医学]