HPLC法测定去痛片中四种成分的含量  

Determination of Four Components in Qutong Tablets by HPLC

作　　者：武谷 钟淮滨 

机构地区：[1]安徽省药品检验所,合肥230061

出　　处：《中国药品标准》2005年第5期31-33,共3页Drug Standards of China

摘　　要：目的:建立一种用HPLC法测定去痛片四种成分含量的方法。方法:采用C18柱,以0.05mol/L磷酸二氢钾溶液(用三乙胺调节pH值为7.3±0.1。)-甲醇(60∶40)为流动相,检测波长215nm,流速为1.0ml/min。结果:样品中四种成分完全分离且线性关系良好,氨基比林、非那西丁、咖啡因和苯巴比妥的加样回收率分别为98.9%、99.9%、99.6%和99.8%(n=9)。结论:本法操作简单,结果准确,可以有效地控制质量。Objective ：To establish a method for determining the components in Qutong Tablets by HPLC. Methods ：Using C18 column,0.05mol/L potassium dihydro potassium phosphate solution （adjusted pH to 7.3 ± 0. 1 by triethylamine）-methanol （60 ： 40） as mobile phase, flow rate was 1.0ml/min,wavelength of detector was 215nm. Results ： Four components were com pletely separated and determined. The average recoveries （n = 9） were 98.9 % for aminopyrine, 99.9 % for phenacetin, 99.6 for caffeine and 99.8% for phenobarbital. Conclusions ：This method is simple and convenient in operation. It is accurate and very suit able for the quality control.

关 键 词：氨基比林 非那西丁 咖啡因 苯巴比妥 HPLC HPLC法 成分含量 去痛片 测定 磷酸二氢钾 加样回收率 C18柱 检测波长 线性关系 

分 类 号：R286.0[医药卫生—中药学] R987[医药卫生—中医学]