Polymerase chain reaction of Au nanoparticle-bound primers  

作　　者：SHEN Hebai HU Min YANG Zhongnan WANG Chen ZHU Longzhang 

机构地区：[1]Life and Environment Science College, Shanghai Normal University, Shanghai 200234, China

出　　处：《Chinese Science Bulletin》2005年第18期2016-2020,共5页

基　　金：supported by the National Natural Science Foundation of China(Grant No.20443005);the Nanotechnology Special Projects of Shanghai(Grant No.0352nm123);the Major Project Foundation of Shanghai Education Administration(Grant No.04DA01).

摘　　要：Polymerase chain reaction (PCR) is a useful technique for in vitro amplification of a DNA fragment. In this paper, a PCR procedure using Au nanoparticle (AuNP) -bound primers was systemically studied. The 5′-SH- (CH2)6-modified primers were covalently attached to the AuNP surface via Au-S bonds, and plasmid pBluescript SK was used as a template. The effects of the concentration of AuNP-bound primers, annealing temperature and PCR cy-cles were evaluated, respectively. The results indicate that PCR can proceed successfully under optimized condition, with either forward or reverse primers bound to the AuNP surface or with both the two primers bound to the AuNP surface. Development of PCR procedure based on AuNPs not only makes the isolation of PCR products very convenient, but also provides novel methods to prepare AuNP-bound ssDNA and nanostructured material.Polymerase chain reaction （PCR） is a useful technique for in vitro amplification of a DNA fragment. In this paper, a PCR procedure using Au nanoparticle （AuNP） -bound primers was systemically studied. The 5＇-SH- （CH2）6-modified primers were covalently attached to the AuNP surface via Au-S bonds, and plasmid pBluescript SK was used as a template. The effects of the concentration of AuNP-bound primers, annealing temperature and PCR cycles were evaluated, respectively. The results indicate that PCR can proceed successfully under optimized condition, with either forward or reverse primers bound to the AuNP surface or with both the two primers bound to the AuNP surface. Development of PCR procedure based on AuNPs not only makes the isolation of PCR products very convenient, but also provides novel methods to prepare AuNP-bound ssDNA and nanostructured material.

关 键 词：聚合酶链反应 PCR 金纳米离子 退火温度 优化条件 

分 类 号：O631.3[理学—高分子化学]